黑鳗藤新苷K的急性毒性及体内抑制细胞和体液免疫的实验研究  

Acute Toxicity Test and Inhibition Effects of Stemucronatoside K on the Cellular and Humoral Immune Responses in Vivo

作　　者：陈峰阳[1] 叶益萍[1] 李晓誉[1] 史红[1] 

机构地区：[1]浙江省医学科学院药物研究所,浙江杭州310013

出　　处：《中华中医药学刊》2009年第3期608-610,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金资助项目(R206439);浙江省医药卫生科技计划资助项目(2006QN004)

摘　　要：目的:研究黑鳗藤新苷K的急性毒性及体内对细胞免疫和体液免疫的抑制作用。方法:采用2,4-二硝基氟苯(DNFB)所致的小鼠迟发型超敏反应(DTH)模型和绵羊红细胞(SRBC)所致小鼠血清凝集素生成模型,观察黑鳗藤新苷K对小鼠体内细胞免疫和体液免疫的抑制作用。结果:黑鳗藤新苷K3.2g/kg剂量内对小鼠无明显毒副作用;10mg/kg以上剂量即可显著降低DNFB诱导的DTH反应小鼠的耳廓肿胀度(P<0.05);20mg/kg以上剂量即可显著减少SRBC所致小鼠血清凝集素抗体的生成(P<0.05)。结论:黑鳗藤新苷K小鼠体内能显著抑制细胞免疫反应和体液免疫反应,无明显毒性。Objective ：To investigate the acute toxicity and the inhibition effects of Stemucronatoside K（SMK） on the cellular and humoral immune responses in vivo. Methods：The immunosuppressive effect of SMK on the cellular immune response was observed by DNFB-induced delayed type hypersensitivity（DTH） in mice;and the experiment of serum agglutinin in mice induced by SRBC was used for observing the inhibiton effect of SMK on the humoral immune response. Resu/ts ：SMK did not show any toxicity under the dose of 3.2g/kg, and significantly inhibited DNFB-induced auricle engorgement of IYFH mice above the dose of 10mg/kg（ P 〈 0. 05 ）, and the production of antibody in the experiment of serum agglutinin in mice above the dose of 20mg/kg（ P 〈 0.05 ）. Conclusion：SMK has marked immunosuppressive effect on the cellular and humoral immune responses in vivo without obvious toxicity.

关 键 词：黑鳗藤新苷K 急性毒性 迟发型超敏反应 血清凝集素 免疫抑制 

分 类 号：R285.5[医药卫生—中药学]