碘杂环化合物IHC-72对心肌细胞慢钙通道的电压和频率依赖性阻滞效应  

Voltage and Frequencydependent Effects of 3,6DimethelaminoDibenzopyriodonium Edetate on Slow Calcium Current in Cardiac Cell

作　　者：陈江斌 李庚山 许家 王利 蒋锡嘉 

出　　处：《湖北医科大学学报》1998年第2期126-129,共4页

基　　金：湖北省教委资助

摘　　要：采用双微电极电压钳制术研究３，６－（二甲氨基）－二苯骈碘杂环依地酸盐（ＩＨＣ－７２）对家兔心室浦肯野纤维慢内向电流（Ｉｓｉ）的电压和频率依赖性阻滞效应。将控制电位（Ｅｃ）置于－５ｍＶ，维持电位（Ｅｈ）分别置于－３５，－３０，－２０ｍＶ时，１０１．６μｍｏｌ／ＬＩＨＣ－７２灌流２０ｍｉｎ前后的峰值Ｉｓｉ分别从７８．７５±１４．３３，４０．６３±５．６３，１８．８±３．５４ｎＡ降至４９．３８±１０．８４，１９．３８±５．６３，２．５±２．６７ｎＡ，抑制率分别为３７．３％，５２．３％，８６．７％（Ｐ均＜０．０１）。用０．１２５，０．２，０．５Ｈｚ频率分别刺激１０１．６μｍｏｌ／ＬΙΗＣ－７２灌流２０ｍｉｎ前后的标本，峰值Ｉｓｉ从灌流前的７５．３８±１４．０２，７５．６４±１３．８６，７４．９８±１３．１２ｎＡ降至５８．７５±９．５４，３４．３８±１０．１６，２１．２５±１１．２６ｎＡ，抑制率分别为２２．１％，５４．５％，７１．７％，Ｐ均＜０．０１。结果提示：ＩＨＣ－７２对心肌细胞慢钙通道具有电压和频率依赖性阻滞效应。Abstract The twomicroelectrode voltage clamp technique was used to study the voltage and frequencydependent effects of 3,6dimethelaminodibenzopyriodonium edetate (IHC72) on slow calcium current (Isi) of isolated rabbit purkinje fibres. As the command potential (Ec) was changed to -5 mV and the holding potential (Eh) changed to -35, -30, -20 mV, 101.6 μmol/L IHC72 reduced the peak Isi from 78.75±14.33, 40.63±5.63 and 18.80±3.54 nA to 49.38±10.84, 19.38±5.03, and 2.50±2.67 nA, reduced by 37.3%, 52.3% and 86.7% respectively in 20 min (P all<0.01). As the stimulation rates were 0.125,0.2, 0.5 Hz, 101.6 μmol/L IHC72 inhibited the peak Isi from 75.38±14.02,75.64±13.86,74.98±13.12 nA to 58.75±9.54, 34.38±10.16, 21.25±11.26 nA, inhibited by 22.1%, 54.4%, 71.7% in 20 min (P all<0.01). The results indicated that IHC72 inhibited Isi in voltage and frequencydependent manners.

关 键 词：药物作用 钙通道 心肌细胞 碘杂环化合物 

分 类 号：R331.31[医药卫生—人体生理学] R972.2[医药卫生—基础医学]