NaCl、光和温度胁迫对龙眼胚性愈伤组织GPX酶活性的影响  

The Effects of NaCl,Light and Temperature on the Activities of GPX of the Embryogenic Callus in Longan

作　　者：陈义挺[1,2] 赖钟雄[1] 李焕苓[1] 何园[1] 林玉玲[1] 邵巍[1] 蔡英卿[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福州350002 [2]福建省农科院果树研究所,福州350013

出　　处：《中国农学通报》2009年第7期149-153,共5页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑计划"台湾果树新品种与品质控制新技术引进与创新"(2007BAD07B01);福建省亚热带果树及特种经济作物种质资源共享平台(2008N2001)资助项目部分内容。

摘　　要：以龙眼胚性细胞系LC2为材料,初步研究了在NaCl、光和温度胁迫下龙眼胚性愈伤组织GPX酶活性的变化规律。试验结果表明:与对照相比,在盐胁迫下短期内GPX酶活性增加,随胁迫时间的延长,低盐胁迫下GPX酶活性先上升后下降,而高盐胁迫下GPX酶活性则呈下降趋势;白光、绿光处理下,随光强的不断加强,GPX活性均呈先上升后下降趋势,且在同样光强下,白光处理的GPX活性均高于绿光;龙眼EC的GPX活性在低温(高温)处理下,与对照相比,随温度不断降低(升高),GPX活性呈先升高后降低趋势。以上结果均表明,在一定逆境胁迫下,细胞内GPX可以起到应答外界刺激的作用,且活性呈规律表达,是植物在逆境胁迫下防御ROS伤害的主要标志之一。In this experiment the Line LC2 of longan embryogenic callus was used as the material for the study on the changing tendency of Glutathione peroxidase （GPX） activities in the embryogenic callus of longan under the stress of salt （NaCl）, light, and temperature. The results showed that： compared with the control, the short-time salt treatment enhanced the activity of GPX, and with the time of stress prolonged, the activities of GPX under low-salt stress treatment ascending first and then descending, while it showed the downtrend under high-salt stress treatment. Under the treatment of the white light and green light, with the light intensity increased, the activities of GPX ascending first and then descending, and when the light intensity was the same, the activities of GPX was higher under the treatment of the white light than the green light. Under the treatment of low-temperature （high-temperature）, with the temperature decreased （increased）, the activities of GPX in the embryogenic callus of longan ascending first and then descending compared with those of the control. The above results indicated that under a certain stress, GPX played a role in response to external stimulation, and had a regular expression, and it was one of the main indicators of ROS injury defense under stress.

关 键 词：龙眼 胚性愈伤组织 GPX NACL 光 温度 

分 类 号：S667.2[农业科学—果树学]