16S rRNA与Vitek-32对临床感染猪肠球菌鉴定结果比较  

Comparison of 16S rRNA and Vitek-32 Identification Results for Enterococcus Isolates from Clinical Swine Infections

作　　者：王亚宾[1] 张红英[1] 陈丽颖[1] 程金平[1] 刘磊[1] 崔保安[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002

出　　处：《中国农学通报》2009年第6期9-12,共4页Chinese Agricultural Science Bulletin

基　　金：郑州市科技攻关项目(083SGYG24123-10)

摘　　要：旨在利用两种常用的细菌分型方法对感染猪的肠球菌的鉴定结果进行比较和分析。将从河南洛阳、济源、许昌、南阳等地送检病猪体内分离的42株革兰阳性球菌纯培养后,分别用16S rRNA基因序列比较和Vitek-32全自动细菌鉴定系统进行鉴定。Vitek-32对42株分离菌中的34株鉴定到种的水平,分别是粪肠球菌(E.faecalis)10株、屎肠球菌(E.faecium)2株、铅黄肠球菌(E.casseliflavus)22株;另有8株未成功鉴定。16S rRNA基因序列分析对42株分离菌成功的鉴定结果是为粪肠球菌12株,屎肠球菌30株。经比较发现,Vitek-32鉴定的10株粪肠球菌中除1株被16S rRNA鉴定为屎肠球菌外,其余9株均相同;Vitek-32鉴定的2株屎肠球菌与16S rRNA鉴定结果相同;Vitek-32鉴定的22株铅黄肠球菌除了1株被16S rRNA鉴定为粪肠球菌外,其余全部被鉴定为屎肠球菌;8株未能被Vitek-32鉴定的肠球菌被16S rRNA方法分别鉴定为粪肠球菌(2株)和屎肠球菌(6株)。在感染猪的肠球菌分型鉴定中,Vitek-32对粪肠球菌鉴定结果与16S rRNA比较具有较高的一致性(75%);而对于屎肠球菌的鉴定结果与16S rRNA比较出现了较大的差异(93.3%)。To compare the two bacteria typing methods as used in Enterococcys identification for swine infection. Forty-two Gram-positive coccal isolates were acquired from infection cases in Luoyang, Jiyuan, Xuchang and Nanyang of Henan Province, and were analysed by means of 16S rRNA gene sequencing and Vitek-32 automatic identification system respectively. 34 out of 42 isolates were determined to species level by Vitek-32, among which were 10 isolates of E. faecalis, 2 of E. faecium, 22 of E. casseliflavus, whereas the other 8 failed to be identified. The 16S rRNA gene sequencing showed that all the isolates were Enterococcus species, with 12 of E. faecalis and 30 of E. faecium. In comparison, among the 10 isolates of E. faecalis by Vitec-32, 9 were verified as the same and the other 1 was identified as E. faecium by 16S rRNA gene sequencing. Both methods showed identical results with the two E. faecium isolates. All the 22 Vitek-32-determined E. casseliflavus were re-decided by 16S rRNA gene sequencing as 1 of E. faecalis and 21 of E. faecium species. The 8 unidentified isolates turned out to be E. faecalis（2） and E. faecium（6）. Vitek-32 results showed good consistence （75%） to that of 16S rRNA gene sequencing when applied to E. faecalis identification, and significant discrepancy（93.3%） occurred in identifying the E. faecium sp isolates.

关 键 词：16SrRNA VITEK-32 肠球菌 鉴定 猪 

分 类 号：S855[农业科学—临床兽医学]