绿色糖单孢菌复合胞外酶漂白纸浆的机理  

Mechanism of Synergistic Bleaching Utilizing Compound Enzyme from Saccharomonospora Viridis

作　　者：张勇[1,2] 蒲俊文[1] 吴玉英[1] 吕卫军[2] 

机构地区：[1]北京林业大学材料科学与技术学院,北京 100083 [2]中国制浆造纸研究院,北京 100020

出　　处：《中国造纸学报》2009年第1期25-30,共6页Transactions of China Pulp and Paper

基　　金：国家林业科技支撑计划(2006BAD18B0502)的资助

摘　　要：以经过绿色糖单孢菌复合胞外酶漂白的纸浆为研究对象,首次采用SEM、GC、FT-IR、CP/MAS13C-NMR、XRD等仪器分析手段全面阐释由一种菌同时产生的两种酶(木聚糖酶和木素过氧化物酶)协同漂白纸浆的作用机理。结果发现该复合胞外酶漂白,对纸浆中的木聚糖和聚阿拉伯糖降解显著;可断裂部分残余木素和木素-碳水化合物复合体,减少或改变纸浆中的发色和助色基团;使纤维结构变得疏松,增强后续漂剂的渗透,促进木素的溶出;漂白对纸浆纤维素的破坏主要集中在无定形区,对结晶区影响较小。The mechanism of synergistic bleaching utilizing compound-enzyme（contain xylanase and lignin peroxidase） from Saccharomonospora viridis was comprehensively studied by using SEM,GC,FT-IR,CP/MAS ^13C-NMR and XRD.The results showed that xylan and poly-arabinose were degraded evidently after treating with compound enzyme.Dissolution of lignin and LCC resulted in a reduction of chromophoric groups in the pulp.After enzyme treatment,fiber structure became loosen and easy to penetrate during the following bleaching sequence.Additionally,XRD illustrated that the enzyme reacted with fiber not in crystalline area but in amorphous area.

关 键 词：绿色糖单孢菌 复合胞外酶 纸浆 协同漂白 机理 

分 类 号：TS745[轻工技术与工程—制浆造纸工程] Q55[生物学—生物化学]