Novel Splice Variants of Bovine Interleukin-1 Receptor-Associated Kinase 2(IRAK2)  

作　　者：WANG Xing-ping LUOREN Zhuo-ma XU Shang-zhong GAO Xue LI Jun-ya REN Hong-yan CHEN Jin-bao 

机构地区：[1]Laboratory of Molecular Biology and Bovine Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing100193. P.R. China [2]Department of Life Sciences ＆ Technology, Xinxiang Medicine University, Xinxiang 453003, P.R. China

出　　处：《Agricultural Sciences in China》2009年第4期508-512,共5页中国农业科学（英文版）

基　　金：supported by the National Key Technology R&D Programs of China (2006BAD01A10,2006BAD14B07,2006BAD04A16);the National High Technology Research and Development Program of China (2006AA10Z197)

摘　　要：Interleukin-1 receptor-associated kinases （IRAKs） are important signaling elements of the toll-like receptors family, which play a role in innate immune responses by coordinating host defense mechanisms. Presently different isoforms of human and murine IRAK2 molecules are cloned, but there is no report on the sequences and structure of bovine IRAK2 gene. In this study, we cloned the bovine IRAK2 gene by RT-PCR and RACE and discovered that there exist two alternative splicing of bovine IRAK2 genes, IRAK2a and 1RAK2b （GenBank accession no. EU528620 and EU528621）. IRAK2a gene is 2 148 bp coding 622 aa, which contains a death domain （aa 14-94） and a kinase domain （aa 205-440）, but 1RAK2b lacks 147 bp of exon 3 corresponding to 1RAK2a, and codes 386 aa which contains only partly kinase domain.Interleukin-1 receptor-associated kinases （IRAKs） are important signaling elements of the toll-like receptors family, which play a role in innate immune responses by coordinating host defense mechanisms. Presently different isoforms of human and murine IRAK2 molecules are cloned, but there is no report on the sequences and structure of bovine IRAK2 gene. In this study, we cloned the bovine IRAK2 gene by RT-PCR and RACE and discovered that there exist two alternative splicing of bovine IRAK2 genes, IRAK2a and 1RAK2b （GenBank accession no. EU528620 and EU528621）. IRAK2a gene is 2 148 bp coding 622 aa, which contains a death domain （aa 14-94） and a kinase domain （aa 205-440）, but 1RAK2b lacks 147 bp of exon 3 corresponding to 1RAK2a, and codes 386 aa which contains only partly kinase domain.

关 键 词：CATTLE IRAK2 alternative splicing 

分 类 号：Q78[生物学—分子生物学] Q55