BFGF及TGF-β1与260例乙肝患者肝脏纤维化及炎症关系研究  

Relationship between the expression of BFGF/TGF-β_1 in liver tissue and liver fibrosis/inflammation in 260 patients with chronic hepatitis B

作　　者：康海燕[1] 董江龙[2] 叶立红[1] 王建彬[1] 刘莲[1] 殷树英[1] 

机构地区：[1]石家庄市第五医院肝病科,河北石家庄050021 [2]河北医科大学第三附属医院,河北石家庄050000

出　　处：《临床肝胆病杂志》2009年第2期97-99,共3页Journal of Clinical Hepatology

摘　　要：目的观察碱性成纤维细胞生长因子(BFGF)及转化生长因子β1(TGF-β1)在慢性乙型肝炎肝纤维化不同进展阶段的表达和定位情况,探讨其与肝纤维化及炎症的关系。方法对260例乙肝患者肝组织BFGF、TGF-β1行免疫组化定量分析,并与肝脏纤维化分期及炎症分级进行统计学分析。结果肝组织BFGF、TGF-β1随肝脏纤维化加重而表达增加,各组与对照组比较及组间两两比较差异均具有统计学意义(P<0.05);且二者与炎症比较,各组与对照组之间差异有统计学意义(P<0.05),但组间两两比较无差异(P>0.05);肝脏BFGF、TGF-β1之间呈正相关,r=0.895(P<0.05)。结论肝组织BFGF、TGF-β1与肝纤维化程度呈正相关,与炎症无确切相关性,BFGF及TGF-β1可作为评价肝纤维化严重程度的重要指标。Objective To observe the immunoloeation and expression of basic fibroblast growth factor （BFGF） and transfor- ming growth factor β1（TGF -β1 ） in different stage on liver fibrosis induced by CHB （chronic hepatitis B） , and to discuss their effects in the development of liver fibrosis and the correlation with hepatitis and fibrosis markers. Methods BFGF and TGF -β1 in the liver of 260 patients with CHB were evaluated with immunohistochemistry and quantitive measurements. The relationship between BFGF/TGF - β1 and staging of hepatic fibrosis/grading of inflammation was analyzed. Results Expression of BFGF and TGF - β1 increased in line with the elevation of fibrotie staging. The levels of BFGF and TGF -β1 in groups of SI - S4 were higher than those in control group SO. The differences were statistically significant （P 〈0. 05）. And there were significant differences between every two groups （ P 〈 0. 05 ）. But in inflammation grading, the surface den- sity in groups G1 - G4 were all higher than that in control group GO （P 〈 0. 05）. But there were no significant differences between every two groups （P 〈 O. 05）. There is close positive correlation between BFGF and TGF- β1 in hepatic tissue, the coefficient was 0. 895 （ P 〈 0. 05）. Conclusion There is a close positive correlation between BFGF/TGF -β1 and hepatic fibrosis, and no correlation with liver inflammation. BFGF and TGF -β1 can be used as the important indicators of the severity of hepatic fibrosis, and furthermore, the understanding of them may provide theoretica basis of anti - fibrosis therapy by antagonists of BFGF and TGF - β1.

关 键 词：乙型肝炎 碱性成纤维细胞生长因子 转化生长因子Β1 肝纤维化 免疫组化 

分 类 号：R512.62[医药卫生—内科学] R575.2[医药卫生—临床医学]