生物素-链霉亲和素-过氧化物酶法快速诊断呼吸道合胞病毒感染  

Rapid Detection of Respiratory Syncytial Virus Antigen by Biotin-Streptavidin-Peroxidase Method

作　　者：项哨[1] 朱圣禾[1] 陶明方[2] 

机构地区：[1]浙江医科大学微生物学教研室 [2]浙江省人民医院儿科

出　　处：《浙江医科大学学报》1998年第2期62-64,87,共4页

基　　金：浙江省卫生厅医学科技研究基金

摘　　要：目的：建立检测下呼吸道感染婴幼儿粘膜细胞呼吸道合胞病毒（RSV）抗原的方法。方法：以生物素标记兔抗RSV抗体，鼻咽部粘膜细胞涂片与该抗体作用后加链霉亲和素-过氧化物酶反应，二氨基联苯胺显色，显微镜下观察结果。结果：RSV抗原总阳性率为41．6％，临床典型和可疑病例抗原检出率分别为47．9％和44．9％，临床不典型者阳性率12．5％（X2=7．9，P＜0．05）。阳性标本中细胞着成棕色，易与阴性标本相区别。结论：本法简便、快速，具有一定敏感性和特异性，适合于基层推广应用。ObjectiveTo establish a method for detecting the respiratory syncytial virus(RSV) infection in theexfoliated epithelial cells of lower respiratory tract in infants and young children. Methods:The rabbit antibodyagainst RSV was labelled with biotin; the streptavidin-Peroxidase and diaminobenzidine were added to cover thesmear of exfoliated respiratory epithelial cells after the reaction of the smear with the biotin-laballed antibody ; the results were examined using a light microscope. Results: The total RSV antigen positive rate was41. 6%; the positive rates in the clinically typical and suspected cases were 47. 9% and 44. 9% respectively ; thepositive rate in the clinically atypical cases was 12. 5 % (X2= 7. 9,p<0. 05). The typical staining pattern exhibited brown color and the positive cells were easy to be differentiated from the negative cells. Conclusion: Themethod is simple and fast with afairly high sensitivity and certain specificity, and especially suitable to be used inthe hospitals at the primary level.

关 键 词：生物素 链霉亲和素 过氧化物酶法 呼吸道 RSV 

分 类 号：R373.1[医药卫生—病原生物学] R446.9[医药卫生—基础医学]