新蒽环类化合物F301A的分离鉴别及活性测定  

Isolation, structure elucidation and determination of bioactivity of F301A

作　　者：杨毓芬[1] 李焕娄[1] 金文藻[1] 李嗣英[1] 

机构地区：[1]中国医学科学院中国协和医科大学医药生物技术研究所

出　　处：《中国抗生素杂志》1998年第2期104-106,共3页Chinese Journal of Antibiotics

摘　　要：从天然无抗菌活性的链霉菌１２５４与变青链霉菌１３２６两者原生质体融合获得的融合子Ｆ３０１的培养液中，经大孔树脂提取、制备型ＴＬＣ（硅胶ＧＦ２５４）、聚酰胺柱层析、ＬＨ－２０柱层析等方法，分离得到一个新结构的蒽环化合物４，６，７，８，９－五羟基－９－乙基－蒽环酮。体内、体外试验均表明其具有免疫抑制作用。A new immunosuppresive agent was isolated from the fermentation broth of the fusant F301A which was obtained by protoplast fusion of antibioticnonproducing strains, Streptomyces sp. 1254 and Streptomyces lividans 1326. By means of extraction with X5 macroreticular resin, TLC, polyamide column and Sephadex LH20 column chromatography a pure sample F301A was obtained. Based on spectral evidences and physicochemical properties the chemical structure of F301A was elucidated as 4,6,7,8,9pentahydroxy9ethyl anthracyclinone which is a novel anthracycline compound.

关 键 词：蒽环化合物 F301A 免疫抑制剂 链霉菌 

分 类 号：TQ465.92[化学工程—制药化工] R979.5[医药卫生—药品]