大鼠脑缺血后海马CA1区脑红蛋白表达的变化及肢体缺血预处理对其影响  

Neuroglobin expression in the CA1 hippocampus after cerebral ischemia and the effect of limb ischemic preconditioning on it in young and aged rats

作　　者：李淑琴[1] 武宇洲 胡玉燕[1] 蔡劲松[1] 张敏[1] 孙晓彩[1] 羡晓辉[1] 李清君[1] 李文斌[1] 

机构地区：[1]河北医科大学病理生理教研室,石家庄050017 [2]河北省心脑血管病研究所

出　　处：《中华老年医学杂志》2009年第4期323-326,共4页Chinese Journal of Geriatrics

基　　金：国家自然科学基金（30770738）;教育部博士点基金（20050089001）;河北省自然科学基金（C200500720、C2008001042）

摘　　要：目的观察青年和老年大鼠脑缺血后海马CA1区脑红蛋白（Ngb）表达的变化及肢体缺血预处理（LIP）对其影响。方法将凝闭双侧椎动脉的青年和老年大鼠均随机分为脑缺血组和脑缺血＋LIP组。采用反转录聚合酶链反应（RTPCR）和Western blot法检测海马CA1区Ngb mRNA和蛋白表达，硫堇染色观察海马CA1区锥体神经元迟发性死亡（DND）情况。结果青年脑缺血组、青年脑缺血＋LIP组、老年脑缺血组、老年脑缺血＋LIP组的Ngb mRNA和蛋白表达分别为0．16±0．02和0．32±0．07、0．52±0．04和0．91±0．06、0．09±0．01和0．22±0．08、0．21±0．01和0．66±0．06。表明老年大鼠脑缺血后海马CA1区Ngb mRNA和蛋白表达较青年脑缺血大鼠降低（P〈0．05），LIP可上调青年和老年大鼠脑缺血后海马CA1区Ngb mRNA和蛋白表达（P〈0．05），但对老年大鼠的上调作用低于青年大鼠（P〈0．05）。硫堇染色显示，海马CA1区神经元密度青年脑缺血组、青年脑缺血＋LIP组、老年脑缺血组和老年脑缺血＋LIP组分别为（38．8±10．9）、（171．5±16．9）、（21．2±12．2）个／mm和（102．7±15．4）个／mm。表明老年大鼠LIP预防脑缺血引起的海马CA1区锥体神经元DND的作用小于青年大鼠。结论老年大鼠脑缺血后Ngb的表达及LIP对其上调作用较青年大鼠明显减弱，这可能是老年大鼠脑缺血后损伤较重和LIP对老年大鼠脑缺血保护作用较弱的原因之一。Objective To investigate the changes of neuroglobin （Ngb） expression in the CA1 hippocampus after cerebral ischemia and the effect of limb ischemic preconditioning （LIP） on it in young and aged rats. Methods SD rats aged 3 months and 21 23 months with permanently occluding bilateral vertebral arteries were randomly divided into cerebral ischemic group and LIP ＋ cerebral ischemic group, respectively. The expression of Ngb mRNA and protein in the hippocampus were investigated by reverse transcription-polymerase chain reaction （RT-PCR） and Western blot methods. The profile of delayed neuronal death （DND） of pyramidal neurons in the hippocampus CA1 was evaluated by using thionin staining under light microscope by determining the neuronal density （ND） and histological grade （HG）. Results Ngb mRNA and protein expressions were 0.16±0.02 and 0. 32±0. 07, 0.52±0.04 and 0. 91±0. 06, 0.09±0.01 and 0.22±0.08, 0.21±0.01 and 0.66± 0.06 in young cerebral ischemia group, LIP ＋ young cerebral ischemia group, aged cerebral ischemia group and LIP ＋ aged cerebral ischemia group, respectively. The expressions of Ngh mRNA and protein after cerebral ischemia for 8 minutes in aged rats were decreased compared with those in the young rats which suffered an identical cerebral ischemia with the aged rats （P〈0.05）. LIP upregulated Ngb mRNA and protein expressions in both young and aged rats which suffered cerebral ischemia （P〈0.05）. However, the up-regulation of Ngb expression in aged rats was significantly less than that in young rats （P〈0.05）. Neuropathological evaluation showed that ND was 38.8±10.9, 171.5±16.9, 21.2±12.2 and 102.7±15.4 in young cerebral ischemic group, LIP ＋ young cerebral ischemie group, aged cerebral iscbemic group and LIP ＋ aged cerebral ischemic group, respectively. It showed that obvious DND of pyramidal neurons was found in young and aged rats after cerebral ischemia. Although LIP effectively protected the pyramidal neurons in the CA1 hippocampus agai

关 键 词：脑缺血 缺血预处理 红蛋白类 海马 

分 类 号：R686[医药卫生—骨科学]