用双水相萃取丙酰螺旋霉素的研究  

Study on the extraction of propionylspiramycin by aqueous twophase system

作　　者：秦德华[1,2] 潘杰[1,2] 高红[1,2] 

机构地区：[1]中国医学科学院中国协和医科大学医药生物技术研究所 [2]中国科学院化工冶金研究所

出　　处：《中国抗生素杂志》1998年第2期144-147,共4页Chinese Journal of Antibiotics

摘　　要：用双水相萃取（ＡＴＰＥ）丙酰螺旋霉素（ＰＲＯ－ＳＰＭ），分别用ＰＲＯ－ＳＰＭ溶液和发酵液，对聚乙二醇（ＰＥＧ）和磷酸盐成相系统进行了研究，选出了合适的成相系统。在研究中发现ＰＲＯ－ＳＰＭ对成相有一定的影响。根据数据提出在ＰＲＯ－ＳＰＭ的双水相萃取过程中疏水作用是影响萃取的主要因素，与蛋白质以静电效应为主不同。用发酵液进行放大试验并与溶媒法进行比较，ＡＴＰＥ法萃取收率提高了３２％，而纯度有所下降。初步探讨了聚合物的重复使用。The extraction technique of propionylspiramycin (PROSPM) by aqueous twophase (ATPE) from the solution or the fermentation broth was discussed. The effect of pH, PEG molecular weight and concentration, salt concentration were investigated in the PEG/phosphate system. The optimum condition was obtained. Results showed that the hydrophobic interaction was the dominant effect contrary to the partition protein by ATPE. The solute itself i.e. PROSPM engaged in the ATPE. Comparing with that of the organic solvent extraction, the recovery was 32% higher by ATPE, but the purity of the sample was somewhat lower. The feasibility of repeated use of PEG was also investigated.

关 键 词：大环内酯类 抗生素 丙酰螺旋霉素 双水相萃取 

分 类 号：TQ465.5[化学工程—制药化工]