香蕉果实乙烯受体基因克隆及其表达特性  

Cloning and Expression Analysis of Ethylene Receptor in Harvested Banana Fruit

作　　者：贺立红[1,2] 陈建业[2] 于伟民[2] 王勇 陆旺金[2] 

机构地区：[1]仲恺农业工程学院生命科学学院,广州510225 [2]华南农业大学园艺学院/广东省果蔬保鲜重点实验室,广州510642 [3]中山市动植物检疫局,广东中山528400

出　　处：《中国农业科学》2009年第4期1359-1364,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金(30571299);广东省自然科学基金(7301741);国家科技支撑计划项目(2006BAD30B06)

摘　　要：【目的】分析香蕉果实成熟及贮藏冷害下乙烯受体基因的表达特性,探讨热处理(38℃,3d)提高果实耐冷性与乙烯受体基因表达的关系。【方法】根据已报道的ETR基因的氨基酸保守序列设计引物,以香蕉果皮总RNA为模板,利用RT-PCR方法克隆香蕉的ETRcDNA,采用Northern杂交分析该基因的表达特征。【结果】香蕉果实在自然成熟进程中,两个基因在果皮和果肉中呈现不同的表达模式:果皮和果肉中Ma-ETR1的表达随果实成熟而逐渐减弱,而Ma-ERS3的表达仅在果实成熟后期略有增强;外源丙烯处理和38℃高温抑制果皮和果肉中Ma-ETR1的表达,却促进了Ma-ERS3的表达;低温促进果皮中Ma-ETR1和果肉中Ma-ERS3的表达,而38℃热处理3d后则能抑制果皮中受低温诱导的Ma-ETR1表达的增强。【结论】外源丙烯和38℃高温正调控Ma-ERS3表达,负调控Ma-ETR1表达;38℃热处理3d提高采后香蕉果实耐冷性与抑制低温诱导的果皮中Ma-ETR1表达的增强有关。【Objective】 To analyze expression profiles of ethylene receptors during fruit ripening and storage at chilling temperature, and investigate the relationship between expressions of ethylene receptors and chilling tolerance induced by heat pre-treatment （38℃ for 3 days）. 【Method】 Using the PCR degenerate primers designed with reference to the conserved amino acid sequences of known ETR, total RNA extracted from banana peel tissues was used as templates, then ETR cDNA fragments were amplified by RT-PCR. Their expression characteristics were studied by the Northern hybridization. 【Result】 The results showed that different expression patterns of two ethylene receptor genes in the banana peel and pulp were observed. Ma-ETR1 expression gradually weakened during fruit ripening while Ma-ERS3 expression accumulated slightly at the later stage of fruit ripening, but the expression of Ma-ETR1 was inhibited. However, the expression of Ma-ERS3 was enhanced by exogenous propylene treatment and high temperature, respectively, in both the peel and pulp tissues. Chilling temperature induced the expression of Ma-ETR1 in the peel and Ma-ERS3 in the pulp, but the accumulation of Ma-ETR1 in the peel tissue induced by chilling temperature was blocked by a heat pre-treatment. 【Conclusion】 These results indicate that the expression of Ma-ERS3 is positively regulated while the expression of Ma-ETR1 is negatively regulated by ethylene or high temperature treatment. Moreover, the inhibition of the accumulation of Ma-ETR1 in the peel tissues induced by chilling temperature is related to the chilling tolerance of harvested banana fruit induced by heat pretreatment.

关 键 词：香蕉 乙烯受体 CDNA克隆 基因表达 果实成熟 耐冷性 

分 类 号：S668.1[农业科学—果树学]