高效液相色谱法测定丹皮酚缓释微球中药物含量  

Content Determination of Paeonol in Delayed-release Microspheres

作　　者：赵红[1] 胡容峰[1] 

机构地区：[1]安徽中医学院药学院安徽省中药研究与开发重点实验室,安徽合肥230031

出　　处：《安徽中医学院学报》2009年第2期52-54,共3页Journal of Anhui Traditional Chinese Medical College

基　　金：安徽省高等学校省级自然科学研究项目(KJ2009A112)

摘　　要：目的：建立自制丹皮酚微球中丹皮酚的含量测定方法。方法：采用Kromasil C18（40mm×250mm．5μm）色谱柱．流动相甲醇-水（52：48），检测波长274nm，流速1．00ml／min，柱温30℃。结果：丹皮酚在0．3124～7．8100ug／ml范围内线性关系良好（r=0．9996），加样回收率为97．11％，RSD=1．56%（n=3）。结论：所建方法简便、结果准确，可用于自制丹皮酚微球的质量控制．Objective： To establish a high performance liquid chromatography （HPLC） method for content determination of paeonol in delayed-release microspheres. Methods： Chromatographic experiments were performed on Kromasil C18 column （40mm×250mm.5μm）. The mobile phase was composed of methanol-water （52： 48）. The detection wavelength was 274 nm. The flow rate was 1.0 ml/min. The column temperature was 30℃. Results： The linear range was 0. 3124-7. 810 μg/ml （r=0. 999 6）. The average recovery was 97.11% with RSD=1. 56% （n=3）. Conclusion： This method is simple, accurate, and suitable for the quality control of delayed-release paeonol microspheres.

关 键 词：丹皮酚 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]