通络方药对糖尿病大鼠胰岛β细胞的保护作用  

Protective effects of Tongluo recipe on damaged beta-cells of rat with streptozotocin-induced diabetes

作　　者：王怀清[1,2] 邹俊杰[1] 苏明丽[3] 雷银雪[3] 邹秀兰[3] 赵瑛[4] 石勇铨[1] 刘先哲[3] 吴以岭[5] 刘志民[1] 

机构地区：[1]长征医院内分泌科,上海200003 [2]宜昌市第一人民医院 [3]湖北宜昌市第一人民医院 [4]长征医院神经内科,上海200003 [5]河北医科大学医药研究院

出　　处：《中国糖尿病杂志》2009年第3期178-180,共3页Chinese Journal of Diabetes

基　　金：国家973资助项目(2005CB523304);长征医院三重三优基金资助项目

摘　　要：目的研究通络方药(TLR)对链脲佐菌素(STZ)所致大鼠胰岛β细胞损伤的保护作用。方法建立糖尿病大鼠模型前8天开始给予TLR直至实验结束。实验结束时测血糖、体重;取胰腺测定胰腺匀浆中丙二醛(MDA)及谷胱甘肽过氧化物酶(GSH-Px)水平,透射电镜检测胰岛β细胞。结果与正常组相比,STZ组胰腺内MDA水平明显升高(P<0.05),GSH-Px活性明显降低(P<0.05),血糖明显升高。TLR预处理明显降低STZ组大鼠的血糖水平,同时降低大鼠胰腺匀浆内MDA含量(P<0.05),升高GSH-Px活性(P<0.05)。透射电镜显示TLR预处理使胰岛β细胞的损伤明显减轻。结论 TLR通过减轻胰腺内氧化应激水平保护胰岛β细胞完整并有效防止STZ诱导的糖尿病发生。Objective To investigate the protective effects of Tongluo Recipe （TLR） on damaged beta-cells of rat with streptozotoein（STZ）-induced diabetes. Methods TLR was given for 8 days prior to induction of diabetes with STZ until the end of the study （4 weeks）. Blood glucose levels and body weights were examined, Malondialdehyde （MDA） and glutathione peroxidase （GSH-Px） activity were measured in pancreatic homogenates at the end of the study. Pancreatic beta-cells were examined by electron microscope. Results STZ induced a significant increase in MDA levels （P〈0. 01） and a marked decrease in GSH-Px activity （P 〈 0.05） in pancreatic tissue. Blood glucose levels were significantly increased in STZ-treated rats only. Pretreatment of STZ-tredated rats with TLR decreased the incidence of diabetes compared with STZ-treated rats without TLR. At the same time, markedly reduced MDA production （P〈 0.05） and elevated GSH-Px activity （P 〈 0. 01） were apparent in the TLR-pretreated rats. Transmission electron microscope observations showed that the STZ-induced beta cell damage was attenuated in rats pretreated with TLR. Conclusions TLR exerts its protective effect on hyperglycemia and beta-cell damage through reducing the oxidative stress and preserving pancreatic beta-cell integrity.

关 键 词：通络方药 链脲佐菌素 Β细胞 氧化应激 

分 类 号：R285.5[医药卫生—中药学]