HPLC法测定冬凌草甲素纳米脂质载体药物的含量及包封率  被引量:17

HPLC determination of content and entrapment efficiency of oridonin nanostructured lipid carriers

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作  者:代文婷[1] 张典瑞[1] 郭晨煜[1] 段存贤[1] 魏玉芝[1] 

机构地区:[1]山东大学药学院药物制剂研究所,济南250012

出  处:《药物分析杂志》2009年第4期587-590,共4页Chinese Journal of Pharmaceutical Analysis

基  金:国家自然基金资助项目(30472130)

摘  要:目的:建立冬凌草甲素纳米脂质载体药物含量及包封率测定的高效液相色谱法。方法:Kromasil C18柱(4.60mm×150mm,5μm);流动相:甲醇-水(52∶48);流速1.0mL·min-1;检测波长:242nm;进样量:20μL;柱温:25℃。建立超滤离心法测定冬凌草甲素纳米脂质载体的包封率。结果:在此色谱条件下冬凌草甲素与辅料及溶剂峰均得到良好分离,冬凌草甲素在1.0~100μg·mL-1浓度范围内线性关系良好(r=0.9998,n=7),冬凌草甲素纳米脂质载体的包封率为(68.2±0.60)%。结论:该方法准确可靠、简单快速,可用于冬凌草甲素纳米脂质载体药物含量及包封率的测定。Objectives:To establish HPLC method to determine the drug content and entrapment efficiency of oridonin nanostructured lipid carriers.Methods:Using methanol-water(52∶48) as mobile phase,the separation was performed on a Kromasil C18 column(4.60 mm×150 mm,5 μm)at a flow rate of 1.0 mL·min^-1.The detection wavelength was 242 nm and the column temperature was 25 ℃.Results:Regression analysis of peak area against oridonin concentration showed good straight line fit over the range examined:1.0^-100 μg·mL^-1 (r=0.9998,n=7).The entrapment efficiency of oridonin nanostructured lipid carriers was (68.2±0.60)%.Conclusions:This method was proved to be simple,accurate,sensitive and applicable for determination of drug content and entrapment efficiency of oridonin nanostructured lipid carriers.

关 键 词:冬凌草甲素 含量测定 包封率 高效液相色谱法 超滤离心法 

分 类 号:R917[医药卫生—药物分析学]

 

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