固相萃取-高效液相色谱法测定小鼠血浆中德氮吡格  

SPE-HPLC determination of tetrazanbigen in mice plasma

作　　者：刘嫱[1,2] 陈志琼[2] 梁艳[2] 杨菲[2] 余瑜[2] 

机构地区：[1]海南医学院药学系药理教研窀,海口571101 [2]重庆医科大学药学院药物化学教研室,重庆400016

出　　处：《药物分析杂志》2009年第4期617-619,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立血浆中德氮吡格检测的固相萃取-高效液相色谱法(SPE-HPLC)。方法:血浆样品经过C18固相萃取小柱净化后,在Lichrospher C18色谱柱(4.6mm×250mm,5μm)上,柱温30℃,以甲醇-0.1%三乙胺水溶液(pH6.5)(90∶10,)为流动相,流速1.0mL·min-1,检测波长260nm,进样量20μL,对德氮吡格进行测定。结果:德氮吡格的血药浓度在1～20μg·mL-1范围内与峰面积呈良好线性关系(r≥0.995);平均回收率为101.4%;高中低3浓度的日内精密度RSD均<4%,日间精密度RSD均<8%,最低定量限为1.0μg·mL-1。结论:该方法简便、准确、可靠,适用于血浆德氮吡格浓度测定及其药代动力学研究。Objective：To develop a solid phase extraction and high-performance liquid chromatographic （SPE-HPLC） method for the determination of tetrazanbigen in plasma with UV-visible detection.Methods：HPLC separation on Lichrospher C18 （4.6 mm×250 mm,5 μm） was carried out with an isocratic mobile phase of 90% methanol in 100 mL methanol-deionized water（pH 6.5）,containing 0.1% triethyl amine and detection at 260 nm.Results：The retention time of tetrazanbigen was less than 6 min.The linearity was good over the concentration range 1-20 μg·mL^-1 （r ≥ 0.995）.The recoveries were in the range 98.1%^-104.5% （n=5）,and the inter-and intra-day RSD were less than 8.0%.The lower limit of quantitation was 1.0 μg·mL^-1.Conclusion：The method is simple,accurate and can be used to determine tetrazanbigen in mice plasma and study of its pharmacokinatics.

关 键 词：德氮吡格 固相萃取-高效液相色谱法(SPE-HPLC) 血浆 

分 类 号：R917[医药卫生—药物分析学]