反相高效液相色谱法测定小鼠血浆中银杏酸浓度  

RP-HPLC determination of ginkgolic acids in mouse plasma

作　　者：杨小明[1] 张秀丽[1] 夏磊[2] 刘方[1] 李永金[3] 

机构地区：[1]江苏大学化学化工学院,镇江212013 [2]江苏大学食品与生物工程学院,镇江212013 [3]江苏大学医学院,镇江212013

出　　处：《药物分析杂志》2009年第4期620-622,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：江苏省社会发展基金(BS2005042);江苏大学高级人才基金(05JDG024);镇江市社会发展基金(SH2006071)

摘　　要：目的:建立测定小鼠血浆中银杏酸的高效液相色谱法。方法:样品预处理以乙腈沉淀蛋白。采用SinoChrom ODS-AP C18色谱柱(250mm×4.6mm,5μm),甲醇-3%醋酸(92∶8)为流动相,流速1.0mL·min-1,检测波长310nm。结果:银杏酸在0.25～50μg·mL-1范围内线性关系良好(r=0.9998),最低检测限为0.08μg·mL-1。提取回收率为87.0%～90.2%(n=5),相对回收率为95.8%～100.1%(n=5),日内RSD≤5.8%(n=5),日间RSD≤9.5%(n=5)。结论:该法灵敏度高,重现性好,精密度和准确性好,适于银杏酸的药代动力学研究。Objective：To develop an HPLC method for determination of ginkgolic acids in mouse plasma.Methods：Plasma samples were treated with acetonitrile for protein precipitation.The analytical column was SinoChrom ODS-AP C18 （250 mm×4.6 mm,5 μm）.The mobile phase consisted of a mixture of methanol-3% acetic acid （92∶8） with a flow rate of 1.0 mL· min^-1.The detection wavelength was at 310 nm.Results：Standard curve was linear in the range of 0.25-50 μg· mL^-1,r=0.9998.The lowest limit of detection was 0.08 μg· mL^-1.The intra-day and inter-day precision were lower than 5.8% and 9.5% （n=5）,respectively.The extraction recoveries were 87.0%-90.2% （n=5） and the method recoveries were 95.8%^-100.1%（n=5）.Conclusion：The method is simple,sensitive,accurate,reproducible and suitable for clinical pharmacokinetic study of ginkgolic acids.

关 键 词：银杏酸 高效液相色谱法 小鼠血浆 

分 类 号：R917[医药卫生—药物分析学]