高效液相色谱法测定大鼠血浆中百草枯的含量  

HPLC determination of paraquat in rat plasma

作　　者：王雷[1,2] 王本杰[1] 孔祥麟[1] 魏春敏[1] 袁桂艳[1] 赵久强[2] 郭瑞臣[1] 

机构地区：[1]山东大学齐鲁医院临床药理研究所,济南250012 [2]新汶矿业集团中心医院药剂科,新汶271233

出　　处：《药物分析杂志》2009年第4期623-626,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立大鼠血浆中百草枯HPLC检测方法,为百草枯中毒患者治疗、预后提供试验依据。方法:样品处理采用35%高氯酸(v/v)沉淀蛋白质。DiamonsilTM C18(250mm×4.6mm,5μm)色谱柱,流动相为0.1mol·L-1磷酸缓冲液(含75mmol·L-1庚烷磺酸钠)-乙腈(88∶12),用三乙胺调pH至3.0,检测波长为258nm。结果:所建立方法在20～5000ng·mL-1范围内线性良好,方法平均回收率为87.5%,日内变异RSD小于10%。大鼠灌胃给50mg·kg-1后8h血浆百草枯浓度为(360.3±130.9)ng·mL-1,至第3d则不能检出。结论:该法准确、灵敏、快速,复杂生物基质中杂质无干扰,适用于血浆中百草枯的分析测定。Objective：To develop an HPLC method for determination of paraquat in rat plasma to provide evidence for the therapy and prognosis of paraquat-poisoned patients.Methods：The rat plasma samples were deproteimized with 35%（v/v） perchlorld acid.A Diamonsil TM C18 column （250 mm×4.6 mm,5 μm） with the mobile phase consisted of 0.1 mol·L^-1 phosphate buffer （75 mmol·L^-1 sodium heptanesulfonate）-acetonitrile （88∶12）,adjusted pH to 3.0 by triethylamine was used to separate paraquat in biological samples,and the detection occurred at 258 nm.Result：A good linearity was obtained in the concentration range of 20-5000 ng·mL^-1,the average recovery was 87.5%,the intra-day precision （RSD） was less than 10%.The concentration of paraquat in rat plasma was （360.3±130.9）ng·mL^-1 after ig. 50 mg·kg^-1 paraquat 8 hours,and paraquat couldn＇t be detected in rat plasma at the third day.Conclusion：The described method was proved to be sensitive,rapid and accurate,can be applied in identification and determination of paraquat in rat plasma.

关 键 词：百草枯 高效液相色谱法 大鼠血浆 

分 类 号：R917[医药卫生—药物分析学]