乳酸脱氢酶对科尔沁细毛羊后代性比的影响  

Effect of Lactate Dehydrogenase on Sex-ratio of Keerqin Fine-wool Sheep

作　　者：冯玉麟[1,2] 刘怡虹[1] 宋伟奇 照格中 赵国藩 朱士恩[2] 

机构地区：[1]内蒙通辽职业学院生化系,内蒙古通辽028046 [2]中国农业大学动物科技学院,北京100193 [3]内蒙民族大学医学院,内蒙古通辽028043

出　　处：《中国畜牧杂志》2009年第7期19-21,共3页Chinese Journal of Animal Science

基　　金：教育部阳光基金(200801N0100238);内蒙古教育厅资助项目(NJ005137)

摘　　要：试验以科尔沁细毛羊为对象,研究了乳酸脱氢酶(LDH)处理对其产羔性别比例的影响。将80只母羊随机分为组LDH处理组和对照组2,然后采用孕酮海绵阴道栓对母羊实施同期发情的第4天开始对处理组按300IU/50kg体重进行LDH处理,每48h注射1次,连续注射3次;待其发情后用鲜精进行人工授精,并对输精后母羊阴道分泌物pH和妊娠后母羊血液中的乳酸脱氢酶比活性及乳酸含量进行测定;同时对LDH处理后其后代的性别比例进行了统计。结果表明:LDH处理组母羊阴道分泌物pH与对照组无显著差异(6.83±0.2vs.7.00±0.2)(P>0.05),但其妊娠母羊血液中总LDH比活性、乳酸浓度及后代雄雌性别比例与对照组均存在显著差异(356.5±137.0、9.64±0.2mmol/L)和0.4∶1vs.157.6±39.5、(1.85±0.2mmol/L)和1.1∶1(P<0.05)。由此得出∶用LDH处理可明显改变妊娠母羊血液中总LDH比活性及乳酸浓度,并可显著提高其后代雌性羔羊比例。The present study were designed to investigate the effect of lactate dehydrogenase （LDH） on the sex-ratio of Keerqin fine-wool sheep. Firstly, eighty sheep were divided into two groups： LPH treated group and the control group. Then each group was treated with CIDR to reach homochronous. From the fourth day after CIDR treatment, LDH solution were injected every 48 h for three times. Sheep were artificial inseminated with fresh semen after oestrum. The pH of vagina secretion were measured with PHS-3C Acidimeter. The total activity of LDH and concentration of lactic acid in blood of pregnant sheep were measured with Markert methord. The sheep pregnancy ratio, sex-ratio were also recorded. The results showed that there were no significant difference in pH of vagina secretion between the two groups （6.83 0.2 vs. 7.00 0.2, P 〉 0.05）. However, the total activity of LDH, concentration of lactic acid in blood and sex radio of lamb in LDH treated group （356.5 137.0, 1.85 0.2 mmol and 0.4：1） differed significantly with those of control group （157.6 39.5, 9.64 mmol 0.2 and 1.1：1） （P 〈 0.05）. In conclusion, LDH treatment could change the the total activity of LDH, concentration of lactic acid in blood and sex radio of lamb.

关 键 词：乳酸脱氢酶 总LDH比活性 乳酸浓度 雄雌性比 科尔沁细毛羊 

分 类 号：S826.2[农业科学—畜牧学] Q959.468[农业科学—畜牧兽医]