猪软骨多糖与H22鼠肝癌细胞共培养物对小鼠体液免疫保护作用的研究  

Humoral immunity effect of co-cultrue of cartilage polysaccharide and H22 cells on murine liver cancer

作　　者：韩雪[1] 刘安军[1] 李研东[2] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津市300457 [2]吉林大学教育部重点实验室人兽共患病

出　　处：《中国饲料》2009年第8期26-28,共3页China Feed

基　　金：国家自然科学基金(20776113);高等学校博士学科点专项科研基金(No.20050057002)

摘　　要：本试验以猪软骨多糖与H22鼠肝癌细胞的共同培养物(PS&H22)为研究对象,探讨其对小鼠体液免疫的影响。结果表明:PS&H22组小鼠的生命延长率和存活率极显著高于模型组(P<0.01);免疫荧光检测结果显示PS&H22免疫小鼠血清可与H22细胞发生特异性结合,说明免疫小鼠体内存在抗H22细胞特异性抗体;ELISA检测表明PS&H22免疫小鼠抗体效价大于12800,进一步证明免疫小鼠血清中抗体含量高,且其抗肿瘤体液免疫得到增强。This experiment was conducted to study the effects of co-cuhure of cartilage polysaccharide and H22 cells by murine liver cancer immunoprophylaxis （PS＆H22） on mice humoral immunity.The results showed that ：the life span rate and survival rate of PS＆H22 immunized group was significantly higher than the others（P 〈 0.01 ）;the specific binding of antiserum and antigen was found by immunofluorescent staining,which confirmed that the anti-H22 cell antibody existed in the immunized mice ;the antibody titer of immunized mice serum was over 12800 detected by ELESA assay.The high antibody titer suggested that the humoral immunity on anticaneer was strengthened.

关 键 词：猪软骨多糖 免疫佐剂 体液免疫 

分 类 号：S816[农业科学—饲料科学]