欧芹素乙拮抗TNF促进牛脑微血管内皮细胞与单核细胞粘附作用  

Inhibitory effects of imperatorin on TNF induced adhesion reaction of BCMEC with monocytes

作　　者：储智勇[1] 龚纯贵[1] 芮耀诚 

机构地区：[1]第二军医大学药学院药理学教研室

出　　处：《第二军医大学学报》1998年第2期156-158,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金

摘　　要：目的：研究肿瘤坏死因子（ＴＮＦ）对体外培养的牛脑微血管内皮细胞（ＢＣＭＥＣ）与单核细胞（ＭＮＣｓ）粘附的影响及欧芹素乙（ＩＭＰ）的拮抗作用。方法：通过测定ＭＮＣｓ中髓过氧化物酶活性的方法，对粘附于ＢＣＭＥＣ单层上的ＭＮＣｓ进行定量。结果：ＴＮＦ在５～２５０Ｕ／ｍｌ时呈剂量依赖性地诱导ＢＣＭＥＣ与ＭＮＣｓ的粘附，ＴＮＦ浓度为１００Ｕ／ｍｌ时促粘附达最大值（６１．０％）。ＴＮＦ与内皮细胞共育１ｈ，即可明显促进粘附作用（５５．７％），２ｈ时达最大刺激效应（６２．８％）。ＩＭＰ在浓度为０．１～１０．０μｍｏｌ／Ｌ时，呈剂量依赖性地拮抗ＴＮＦ诱导的粘附作用，在１０．０μｍｏｌ／Ｌ时抑制率达９０．３％。Objective: To study inhibitory effect of imperatorin(IMP) on tumor necrosis factor (TNF) induced adhesion reaction of cultured bovine cerebromicrovascular endothelial cell (BCMEC) with monocytes in vitro.Methods:The assay was based on the measurement of the myeloperoxidase activity in monocytes that had adhered to BCMEC layers.Results:At concentrations from 5 to 250 U/ml,TNF was shown to induce adhesion reaction of cultured BCMEC with monocytes in a dosedependent manner.The effect of TNF reached maximum (61.0%) at 100 U/ml.After 1 h incubation of BCMEC,addition of TNF significantly stimulated the adhesion reaction of BCMEC with monocytes(55.7%).After 2 h incubation the effect reached maximum(62.8%),then the effect slightly decreased.At concentrations from 0.1 to 10.0 μmol/L,IMP was found to antagonize induced adhesion reaction.Its maximal rate of inhibition was 90.3% at 10.0 μmol/L.Conclusion:IMP may decrease the TNF induced adhesion reaction of BCMEC with monocytes.

关 键 词：内皮细胞 单核细胞 IMP 动脉粥样硬化 TNF 

分 类 号：R543.505[医药卫生—心血管疾病]