不同重悬细胞法在洗涤过程中对水牛外周血单个核细胞活率的影响  

Influence of Buffalo PBMC Activity by Different Resuspension Methods During Washing Process

作　　者：韩忠燕[1] 张宝红[1] 黄海愉[1] 张为宇[1] 黄维义[1] 钟恒禄[1] 

机构地区：[1]广西大学动物科学技术学院,南宁530005

出　　处：《中国畜牧兽医》2009年第4期100-102,共3页China Animal Husbandry & Veterinary Medicine

基　　金：广西自然科学基金项目:水牛单核-巨噬细胞系的建立及大片吸虫产物对细胞系功能的影响(桂科自0832036);国家自然科学基金项目:水牛单核-巨噬细胞系的建立和鉴定及大片吸虫功能分子对细胞系功能调节作用的研究(30860204)

摘　　要：为了探讨在离心洗涤水牛外周血单个核细胞(peripheral blood mononuclear cell,PBMC)过程中以移液器吹打法和晃动离心管法重悬细胞对其活率的影响。对经密度梯度离心后初步收集到的PBMC,分别用移液器吹打和晃动离心管2种方法重悬细胞,离心洗涤后对细胞活率进行计数。结果发现,移液器吹打法得到的细胞活率为(74.9±2.6)%,而晃动离心管法使细胞活率达到(98.5±0.5)%,差异极显著(P<0.01)。说明在洗涤PBMC时,晃动离心管法重悬细胞能大大提高细胞活率,可为那些对细胞活率要求高的试验提供一个较为有效的方法。The peripheral blood mononuclear cell （PMBC） activity of buffalo was comported by different resuspension methods during washing process. The PBMC was separated by density gradient centrifugation, then cell was resuspended by transferpettor-spirting method ＆ centrifuge tube-sloshing method, when cell was washed three times the activity was evaluated. The cell activity by transferpettor-spirting method ＆ centrifuge tube-sloshing method was（74. 9 ±2.6）% and（98. 5 ± 0.5）% respectively, the difference of cell activity was significant（P〈0.01）. The activity of PBMC was higher by centrifuge tube-sloshing, which could provide an useful method for further study that requiring higher cell activity.

关 键 词：水牛外周血单个核细胞 重悬细胞法 细胞活率 

分 类 号：S823.83[农业科学—畜牧学]