番茄灰霉病生防细菌BAB-1的鉴定及发酵条件的优化  

Identification of Biocontrol Bacterial Strain BAB-1 against Tomato Grey Mold and Optimization of Fermentation Conditions

作　　者：刘宁[1,2] 郭庆港[2] 安海 李社增[2] 鹿秀云[2] 马平[2] 董金皋[1] 

机构地区：[1]河北农业大学植物保护学院,河北保定071001 [2]河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,河北保定071000 [3]河北省易县农业开发办公室,河北易县074200

出　　处：《中国农业科技导报》2009年第2期56-62,共7页Journal of Agricultural Science and Technology

基　　金：国家863计划项目(2006AA10A211);河北省农林科学院财政专项(2007055001);“十一五”国家科技支撑计划项目(2006BAD08A02)资助

摘　　要：菌株BAB-1是从土壤中分离出的对番茄灰霉病具有较好防治效果的拮抗细菌。通过对该菌株进行形态特征观察、16SrDNA序列分析和API50CHB细菌鉴定试剂盒鉴定,确定菌株BAB-1为枯草芽孢杆菌(Bacillussubtilis)。采用16种培养基对菌株BAB-1进行了发酵培养,结果表明,3号培养基最适合该菌株菌体生长和抑菌物质的产生。进一步进行了培养条件的优化,发现在培养温度30℃,转速210rpm,初始pH7.24,接种量3%,装样量为70mL/250mL时菌体生产量最高,菌量达到1.63×109cfu/mL;而在培养温度30℃,转速210rpm,初始pH7.24,接种量2%,装样量为100mL/250mL时最适合抑菌物质的产生,对番茄灰霉病菌的抑菌圈直径达到1.81cm。Antigonistic bacterium BAB-1 was isolated from soil and showed potential for controlling tomato grey mold. Strain BAB-1 was identified as Bacillus subtilis according to its morphological characteristics, 16S rDNA sequence and the result of API 50CHB system. 16 different media for the bacterial growth and production of antifungal substrates were tested. Results showed that the medium No. 3 was suitable for either bacterial growth or antifungal substrates producing. Optimum fermentation conditions for bacterial growth were as following： temperature 30℃, 210 rpm, pH 7. 24, inoculation amount 3% and amount of loading 70 mL/250 mL flask. Under these conditions, the cell density could reach 1.63×10^9 cfu/mL. While the optimum conditions for antifungal substance producing were temperature 30℃, 210 rpm, pH 7.24, inoculation amount 2% and amount of loading 100 mL/250 mL flask. Under these conditions, the inhibition zone of strain BAB-1 against Botrytis cinerea could reach 1.81cm width.

关 键 词：灰霉病菌 枯草芽孢杆菌 鉴定 培养基优化 生防细菌BAB-1 

分 类 号：S476[农业科学—农业昆虫与害虫防治]