在神经干细胞中Sox2直接调节Olig2的转录活性  

Sox2 directly activate Olig2 transcription in neural stem cells

作　　者：扈启宽[1] 李美育[2] 李学国 徐方[1] 孙涛[1] 

机构地区：[1]宁夏医科大学,宁夏银川750004 [2]北京大学干细胞研究中心,北京100191 [3]辽宁朝阳双塔疾控中心,辽宁朝阳122000

出　　处：《宁夏医学杂志》2009年第4期300-301,F0002,共3页Ningxia Medical Journal

基　　金：宁夏自然科学基金(编号NZ0777;NZ0778;NZ0784)

摘　　要：目的研究Sox2对Olig2直接转录调节。方法荧光素酶报告基因法(Luciferase assay)检测Sox2对Olig2基因启动子激活作用;染色质免疫共沉淀法(CHIP assay)验证体外培养的神经干细胞中Sox2与Olig2基因启动子DNA的结合位点。结果在神经干细胞中,Sox2转录因子与Olig2基因启动子DNA存在结合(-1232-977,ATG为+1),并能在体外激活Olig2的转录。结论Sox2可直接调节Olig2基因的转录活性,参与干细胞向少突胶质分化潜能的维持。Objective To study the binding of Sox2 with Olig2 promoter and the activation of the Olig2 transcription. Methods To test the activation of Olig2 - promotor by Sox2 through luciferase assay and to examine the actual binding of Sox2 and Olig2 - promotor DNA in cultured neural stem cells by the CHIP assay. Results Sox2 could increase the relative luciferase activatity of Olig2 - promotor in a dose dependent manner bind to Olig2 - promotor DNA at the - 1232 - - 977 sequence. Conclusion Sox2 activates the transcription of Olig2 geue and therefore take part in maintaining of the differentiating potentiality into oligodendrocytes.

关 键 词：SOX2 OLIG2 荧光素酶报告基因法 染色质免疫共沉淀 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]