大鼠肌肉中微生物ATP含量及其在死亡时间推断中的应用  

Microbial ATP in Rat Muscle Tissue and Its Application for the Estimation of Postmortem Interval

作　　者：刘茜[1] 翁义星[2] 杨帆[1] 郑娜[1] 刘良[1,3] 

机构地区：[1]华中科技大学同济医学院法医学系,湖北武汉430030 [2]福州市公安局刑侦支队,福建福州350011 [3]中国政法大学证据科学教育部重点实验室,北京100040

出　　处：《法医学杂志》2009年第2期81-84,共4页Journal of Forensic Medicine

基　　金：湖北省卫生厅科研基因项目资助(JX2B02)

摘　　要：目的研究死后大鼠肌肉组织中微生物ATP浓度变化及其与死亡时间(postmortem interval,PMI)的关系。方法取健康SD大鼠处死,死后不同时间取肌肉组织,用生物发光法检测微生物ATP浓度,对所得数据进行统计学处理。结果死后肌肉组织中微生物ATP含量随时间延长而增加,于7d达到高峰,随后逐渐降低,并于10d再次略微升高。PMI在0～9d内与肌肉中微生物ATP含量变化拟合最优。以PMI为自变量的三次多项式回归方程为:y=0.020x3-0.166x2-0.666x+13.412(r2=0.989,P<0.01)。结论死后肌肉组织中微生物ATP含量变化可用于PMI推测;本检测方法所需检材量小,不受组织自身降解的影响。Objective To study the relationship between the changes of microbial ATP in rat muscle tissue and the postmortem interval（PMI）. Methods Healthy SD rats were sacrificed and their muscles were sampled at different postmortem intervals. The concentration of microbial ATP was detected using bioluminescent assay and the data was statistically analyzed. Results The content of microbial ATP in rat muscle tissue increased along with PMI extension and peaked on postmortem day 7, thereafter decreased gradually, but increased slightly on postmortem day 10 once again. The PMI correlated best with the content of microbial ATP in rat muscle tissue within 9 days. If the PMI was the independent variable, the cubic polynomial regression equation was y=0.020x^3-0.166x^2-0.666x＋13.412（r2^=0.989, P〈0.01）. Conclusion Changes of microbial ATP content in rat muscle tissue may be used for estimation of PMI. Since this assay requires only small amount of tissue with much less influence by self-decomposition, it may broaden the time range of PMI estimation.

关 键 词：法医病理学 腺苷三磷酸 发光测定法 死亡时间 微生物 

分 类 号：R711.6[医药卫生—妇产科学] R339.14[医药卫生—临床医学]