HPLC法测定粉背雷公藤中雷公藤甲素的含量  

Determination of triptolide in Tripterygium hypoglaucum by HPLC

作　　者：陈岳祥[1,2] 乔艳[1] 乔化民[1] 姚建忠[2] 

机构地区：[1]中国人民解放军第181医院药剂科,广西桂林541002 [2]第二军医大学药学院,上海200433

出　　处：《药学实践杂志》2009年第2期134-136,共3页Journal of Pharmaceutical Practice

基　　金：广西科技攻关与新产品试制项目(桂科攻NO.0719006-3-9)

摘　　要：目的：建立高效液相色谱法测定广西产粉背雷公藤中不同部位雷公藤甲素的含量方法。方法：采用C18色谱柱（250mm×4．6mm，5μm），流动相为甲醇-水（45：55），检测波长217nm，流速1mL／min，柱温24℃，进样量为20μL。结果：雷公藤甲素在0．5～10μg／mL范围内呈良好的线性关系；平均加样回收率为99．61％，RSD为1．69％（n=5）；粉背雷公藤根部和茎枝中雷公藤甲素的平均含量分别为14．98和3．64μg/g。结论：本方法简便可行、定量准确、重现性好，可用于粉背雷公藤中雷公藤甲素的质量控制。Objective： To develop a HPLC method for determinating content of triptolide in different parts of Tripterygium Hypoglaucum（ Levl. ）Hutch from Guangxi in China. Methods： The determination of triptolide was performed by reverse-HPLC on C18 column （250 mm× 4.6 mm） using methanol-water（45： 55） as mobile phase with a flow rate of 1 mL/min. The detecting wavelength was at 217 nm and the injection volume was 20 μL. Results ： The linear range of triptolide was 0.5 - 10 μg/mL （ r = 0. 999 9 ）. The average recovery was 99.61% , and RSD was 1. 68%. The average content of triptolide in Tripterygium Hypoglaucum （ Levi. ） Hutch was 14.98 μg/g in root and 3.64 μg/g in stem. Conclusions ： The established method is simple, convenient, aecurate, reproducible, and can be applied to the quality control of triptolide in Triterygium hypoglaucum（ Levl. ） Hutch.

关 键 词：粉背雷公藤 雷公藤甲素 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]