白眉蝮蛇蛇毒纤溶酶的分离纯化  

Purification of Fibrinolytic Enzyme from Agkistrodon Halys Ussriensis Emelianor Venom

作　　者：金星光[1] 黄寓[1] 关书博[1] 王珏[1] 

机构地区：[1]辽宁省血栓病中西医结合医疗中心实验室,辽宁沈阳110101

出　　处：《中国药业》2009年第8期23-23,共1页China Pharmaceuticals

摘　　要：目的用白眉蝮蛇蛇毒分离纯化纤溶酶。方法利用离子交换层析、亲和层析、分子筛层析技术进行分离纯化纤溶酶。结果得到了电泳纯的纤溶酶,其相对分子质量为24000。结论该工艺可以快速地分离纯化白眉蝮蛇蛇毒纤溶酶。Objective To purify fibrinolytic enzyme from Agkistrodon halys ussriensis Emelianor Venom. Methods Fibrinolytic enzyme was purified by ion exchange chromatography, affinity chromatography and gel filtration chromatography. Results The purified fibrinolytic enzyme showed one band in SDS- PAGE with relative molecular weight 24 000. Conclusion The process can be used to purify rapidly fibrinolytie enzyme from Agkistrodon halys ussriensis Emelianor Venom.

关 键 词：蛇毒 纤溶酶 分离 纯化 

分 类 号：R284.2[医药卫生—中药学] R282.74[医药卫生—中医学]