针灸预刺激对AD大鼠脑内自由基影响的实验研究  

Experimental Research of Acupunctural Pre-stimulation on Changing the Level of Cerebral Radicals of AD Rats

作　　者：柯红[1] 孙国杰[1] 周华[1] 

机构地区：[1]湖北中医学院

出　　处：《湖北中医学院学报》2009年第2期14-16,共3页Journal of Hubei College of Traditional Chinese Medicine

基　　金：国家自然科学基金;[30772837]

摘　　要：目的观察针灸预刺激"百会"、"肾俞"穴对阿尔茨海默病(AD)大鼠脑内丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-Px)活性的影响。方法Aβ25-35注入SD大鼠大脑海马区造模,造模前后采用灸、针、针加灸方法进行治疗,并设正常组、假手术组和模型组。应用分光光度计测定大鼠大脑海马中MDA含量及GSH-Px活性的变化,并与模型组进行比较。结果针灸预刺激可降低模型动物MDA含量(P<0.05,P<0.01),提高GSH-Px活性(P<0.05,P<0.01)。结论针灸能清除体内自由基,提高机体的抗氧化能力,减轻脑内自由基对神经元的损伤,从而起到延缓脑老化的作用。Objective To investigate, in the light of radical theory, influences in cerebral contents of malondialdehyde （MDA） and gluta- thioneperoxdaso （ GSH - Px） activity by acupunctural pre - stimulation at＂ Baihui＂ （ DU 20） and ＂Shenshu＂ （ BL 23 ） aeupoint in rat models of Alzheimer＇ s Disease. Method Changes of cerebral contents of malondialdehyde （MDA） and glutathioneperoxdaso （ GSH - Px） activity were determined by spectrophotometry. Results The acupunctural pre - stimulation decreases MDA content and enhances GSH - Px aeitivity in model animals. Conclusion Acupuncture treatment delays brain - aging via attenuating neuronal damage caused by cerebral free radicals due to the enhancement of body antioxidative capability by eliminating free radicals.

关 键 词：阿尔茨海默病 针灸疗法 自由基 丙二醛 谷胱甘肽过氧化物酶 

分 类 号：R245[医药卫生—针灸推拿学]