禾谷镰孢菌微管相关蛋白基因(map)克隆及其与多菌灵抗药性关系分析  

Cloning of microtubule-associated proteins gene(map) from Fusarium graminearum and analyzing its relationship with carbendazim-resistance

作　　者：陈长军[1] 李俊[1] 于俊杰[1] 王建新[1] 周明国[1] 

机构地区：[1]南京农业大学植物保护学院,江苏南京210095

出　　处：《南京农业大学学报》2009年第1期160-163,共4页Journal of Nanjing Agricultural University

基　　金：国家973项目(2006CB101900);国家自然科学基金项目(30671048;30671348);教育部高等学校博士点基金项目(20050307034);江苏省科技攻关项目(BG2006328);国家863计划项目(2008AA10Z414)

摘　　要：根据禾谷镰孢菌(Fusarium graminearum)测序菌株NRRL31084(PH-1)的微管相关蛋白基因(map)核苷酸序列设计4对引物,采用PCR方法测定了禾谷镰孢菌对多菌灵(MBC)不同敏感性表型的6个中国菌株的map全序列。DNA序列比对表明,中国的3个敏感菌株和3个抗药菌株的map核苷酸序列相似性没有差异,表明多菌灵抗药性与微管相关蛋白无关。该基因全长1 793 bp,含有6个内含子,编码199个氨基酸;与NRRL31084的map核苷酸序列相似性为99.39%,存在10个差异核苷酸,与其所编码的氨基酸序列相似性为100%。The full-length nucleotide sequence of microtubule-associated proteins gene （map） from each of 6 Fusarium graminearum strains from China which had different carbendazim （MBC） sensitivity phenotypes were cloned using PCR with 4 primer pairs designed in accordance with nucleotide sequence of map from the genome-sequenced isolate, NRRL31084 （PH-1）. The DNA sequence comparison showed that there was no difference in the nucleotide sequence of map among 3 sensitive and 3 resistant strains from China. This result demonstrated that there was no relationship between MBC-resistance and map gene. The full-length of the gene spans 1 793 bp, including 6 introns, encoding 199 amino acids. With 99.39% homology, there were 10 nucleotide differences in map gene between PH-1 isolate and the 6 strains from China. The homology of the deduced amino acid sequence of the gene was 100% between the 6 strains and PH-1 isolate.

关 键 词：禾谷镰孢菌 微管相关蛋白基因(map) 多菌灵 抗药性 

分 类 号：S482.2[农业科学—农药学]