栗疫病菌单个营养体不亲和性基因差异对弱毒性病毒传递的影响  

Effects of one different vic genes on transmission of hypovirus in Cryphonectria parasitica

作　　者：李涛[1] 华朝晖[2] 林少波[2] 兰春能[2] 许利强[1] 乔广行[1] 邓清超[1] 燕娜娜[1] 王克荣[1] 

机构地区：[1]南京农业大学植物保护学院,江苏南京210095 [2]浙江省遂昌县林业技术推广总站,浙江丽水323300

出　　处：《南京农业大学学报》2009年第2期65-69,共5页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(30470062);浙江省重大科技专项(优先主题)农业项目(2008C12067-2)

摘　　要：研究了栗疫病菌(Cryphonectria parasitica)菌株间单个营养体不亲和性(vegetative incompatibility,vic)基因差异对弱毒性病毒传递的影响。将含病毒菌株与具单个vic基因座位差异的野生型菌株配对培养,10 d后调查菌株间的病毒传递率。结果表明,相同vic基因型菌株间的病毒传递率为100%;在1个vic座位上的基因不同通常会降低病毒的传递率,在vic-Cc座位上基因差异菌株间的病毒平均传递率为85.93%,而在vic-Dd座位上基因不同菌株间病毒的平均传递率仅为10.30%,在vic-Aa、vic-Bb座位上基因不同的菌株间病毒平均传递率分别为44.12%、47.93%。vic-Bb座位上基因不同菌株间的病毒正反向传递率相差非常大,由vic-b菌株向vic-B菌株的平均病毒传递率为83.13%,而从vic-B菌株向vic-b菌株的平均病毒传递率只有23.15%;其他3个vic座位上基因不同菌株间的病毒传递率也具有方向性,但不明显。Effect of heteroallelism at each vegetative incompatibility （vic） locus on transmission of hypovirus in Cryphonectria parasiitica was characterized. The 16 isolates of different genotype at 4 vic loci were obtained through sexsual crossing between isolates which have known genotypes. Virus transmission was assayed by growing donor and recipient isolates together. Virus transmission occurs with 100% frequency when donor and recipient isolates have the same vic genotypes but heteroallelism at one of these loci generally reduced virus transmission. Transmission occurred in 85.93% of pairings that were heteroallelic at vic-Cc while only in 10. 30% of pairings that were heteroallelic at vic-Dd. The average virus transmission rates were 44. 12% and 47.93% respectively when vic-Aa and vic-Bb were heteroallelic. Virus transmission was asymmetric, at the extremes, pairs that were heteroallelic at vic- Bb showed strong asymmetry： transmission rate was 83. 13% when virus transfer from vic-b isolate to vic-B but occurred only 23. 15% when virus transfer from vic-B isolate to vic-b, and in other vic loci this asymmetry were also observed though not significantly.

关 键 词：栗疫病菌 营养体不亲和性 vic基因 传递 弱毒性病毒 

分 类 号：S432.44[农业科学—植物病理学] S763.15[农业科学—农业昆虫与害虫防治]