一种简便可靠的兔肾移植模型的建立  

Establishment of a simple and reliable rabbit model of renal allograft transplantation

作　　者：曾春林[1] 肖序仁[1] 郁华亮[2] 

机构地区：[1]解放军总医院泌尿外科,北京100853 [2]解放军总医院第304临床部泌尿外科,北京100037

出　　处：《军医进修学院学报》2009年第2期213-214,217,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：罗氏基金:免疫耐受的机制

摘　　要：目的:建立一种简便可靠的兔肾移植模型。方法:用新西兰白兔作为供体和受体。供受体腹主动脉和下腔静脉分别行端--侧吻合;输尿管带膀胱瓣与膀胱吻合。结果:新西兰白兔肾移植模型稳定;肾移植组兔术后第2天起,血清肌酐浓度(SCr)迅速上升,从术前(179.76±28.36)μmol/L上升到术后的(693.55±36.49)μmol/L,而对照组血清肌酐浓度较稳定;一般情况观察和移植肾外观形态检查均出现典型急排反应。结论:新西兰白兔肾移植模型制作简便、可靠,可用于移植免疫学研究。Objective： To establish a simple and reliable rabbit model of renal allograft transplantation. Methods： Newzealand white rabbits were used as the donor and recipient. End-to-side anastomosis of the donor＇s inferior vena cava to reeipient＇s inferior vena cava was performed. End-to-side anastomosis of the donor＇s abdominal aortas to recipient＇s abdominal aortas was performed. Urinary tract reconstruction was accomplished by suturing the donor＇s ureter with a bladder patch to the recipient＇s bladder. Results： The rabbit model of renal allograft transplantation was reliable. The serum creatinine （SCr） level in- creased from （ 179.76 ± 28.36 ） μmol/L to （ 693.55 ± 36.49 ） μmol/L in transplantation group after surgery. The serum SCr level remained stable in the control group. Typical changes of acute renal rejection occurred in the rabbit model of renal allograft transplantation. Conclusion： Rabbit model of renal allograft transplantation is simple and reliable, and thus can be used as a model to study transplantation immunity.

关 键 词：兔 肾移植 模型 动物 

分 类 号：R617[医药卫生—外科学]