离子束介导玉米DNA的水稻变异后代AFLP分析  

AFLP ANALYSIS OF RICE TRANSFORMED WITH MAIZE DNA BY PARTICLE BEAM

作　　者：姬生栋[1] 陈鹏[1] 王加传[1] 袁召[1] 岳春辉[1] 王知丰[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《核农学报》2009年第2期197-202,共6页Journal of Nuclear Agricultural Sciences

基　　金：河南省自然科学基金(082300430360);河南省国际科技合作项目(0646620016)

摘　　要：利用离子束介导法将玉米(郑单14)DNA片段转入水稻豫粳6号,经过5年的筛选得到了基本稳定遗传的水稻变异株系。本研究通过对64对AFLP选扩引物的筛选,优选出了18对选扩引物,它们能同时在2个变异株系中扩增出大量的DNA指纹条带,并且同时能扩增出差异带及"目的带"。用AFLP分子标记初步分析了2个变异株系与对照间的差异,变异株系中出现了新带、缺失带和"目的带"等几种情况,这些DNA水平上的差异,表明离子束介导玉米DNA育成的水稻材料可能已经插入了玉米的DNA。Many stable heritable rice lines were obtained via five years agricultural selection, which were derived from rice （Oryza stative Japonica） Yujing-6 transgened with large fraction DNA of Zhengdan-14 （zea mays L. ） by particle beam method. 18 pairs optimum selective primers were got by screening from 64 pairs AFLP selective primers via experiment on two mutant lines, which could amplify many DNA fingerprints and also could amplify polymorphic bands and target bands, both in this two mutant lines. Then the two mutant lines and two controls were analyzed with AFLP, the results showed that many polymorphic bands （such as novel bands, target bands, missing bands） were found in mutant lines. The discrepancy in DNA level indicated that rice, transgened with large fraction DNA of Zhengdan-14 by particle beam, might be inserted maize DNA and inherited steadily in some degree. It also indicated that it was possible to cultivate novel rice variety transformed with wide DNA by particle beam.

关 键 词：离子束 水稻 AFLP 玉米DNA 

分 类 号：S511[农业科学—作物学]