N^＋离子注入法选育高产葡萄糖耐量因子(GTF)酵母菌株  

SCREENING OF HIGH-YIELD GTF YEAST BY N^+-IMPLANTATION

作　　者：高艳红[1,2] 吕加平[1] 刘鹭[1] 李淑荣[1] 

机构地区：[1]中国农业科学院农产品加工研究所/农业部农产品加工与质量控制重点开放实验室,北京100193 [2]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018

出　　处：《核农学报》2009年第2期235-238,共4页Journal of Nuclear Agricultural Sciences

基　　金：国家航天育种工程项目(2006HP20013)子课题

摘　　要：本研究通过铬耐量筛选的方式,从12株供试啤酒酵母中获得1株高耐铬性啤酒酵母菌株,并对该菌株进行N+注入诱变,注入能量为50keV,注入剂量1×2.6×1013、2×2.6×1013、3×2.6×1013、4×2.6×1013、5×2.6×1013和6×2.6×1013ion/cm2。结果表明,最适诱变的注入剂量为4×2.6×1013ion/cm2,得到1株高产GTF的啤酒酵母菌株M11-1A11,其富铬能力较诱变前提高了22.4%。经5次传代发酵培养,突变株的富铬性能稳定。In this study, one of the highest chromium-resistant strain was screened from 12 tested brewer＇s yeast N^＋ ion implantation was used to mutate this yeast and screened high-yield GTF yeast strains with Cromium tolerance method. The mutagenesis was conducted by 50 KeV N^＋ ion implantation with the doses of 1 × 2.6 × 10^13 ,2 × 2.6 × 10^13 ,3 × 2.6× 10^13 ,4 × 2.6 × 10^13 ,5 × 2.6 × 10^13 and 6 × 2.6 × 10^13ion/cm^2 . Results showed that the optimum dose was 4 × 2.6 × 10^13icon/cm^2 , and a strain M11-1 A11 of high-producing GTF was obtained. Its organic Cr content was increased by 22.4 % than the original strain. Its fermentation property was stable after 5 generation transfer inoculation.

关 键 词：N^+离子注入 GTF 富铬酵母菌 筛选 

分 类 号：Q933[生物学—微生物学]