切花月季无性系60^Co γ射线离体诱变研究  

MUTAGENESIS OF THE SOMACLONES in vitro OF CUT ROSES BY ^(60)Co γ-RAYS IRRADIATION

作　　者：瞿素萍[1,2] 唐开学[2] 王继华[2,3] 苏艳[1,2] 王丽花[1,2] 张颢[3] 

机构地区：[1]云南省农业科学院质量标准与检测技术研究所,云南昆明650205 [2]农业部花卉产品质量监督检验测试中心,云南昆明650205 [3]云南省农业科学院花卉研究所,云南昆明650205

出　　处：《核农学报》2009年第2期239-243,共5页Journal of Nuclear Agricultural Sciences

基　　金：云南省自然科学基金项目(2005C0037Q);云南省科技攻关项目(2006NG14);云南省人才培引项目(2006PY04)

摘　　要：以月季叶片愈伤组织和再生的不定芽2种材料为外植体,对其体细胞无性系进行离体辐射诱变,研究了^(60)Coγ辐照对叶片愈伤组织再生率、不定芽增殖率及后期生长情况的影响。结果表明,^(60)Coγ射线处理大大降低了叶片愈伤组织的再生频率,叶片再生不定芽更适宜作为离体辐照诱变材料。不定芽的适宜诱变剂量为50～60Gy,由回归方程导出其致死剂量为122Gy,半致死剂量约为76Gy。Mutagenesis of cut rose in vitro irradiated by 60^Co γ-rays was studied. The callus of leaves and regenerations adventitious bud was used as the explants for mutagenesis. Effect of 60^Co γ-rays irradiation on the callus＇s regeneration rate, adventitious bud＇s multiplication rate and vegetal status were studied. The results showed that the regeneration frequency of callus was decreased by 60^Co γ-rays irradiation. The regenerations adventitious bud was the better experimental materials compared with the callus of leaves. The lethal dose was 122Gy and the semi-lethal dose was 76Gy according to the regression equation. The appropriate dose on adventitious bud by irradiation rays was 50 - 60Gy.

关 键 词：月季 无性系 60^Co Γ射线 辐照诱变 

分 类 号：S685.12[农业科学—观赏园艺]