Identifying Chinese species of Gammarus(Crustacea:Amphipoda) using DNA barcoding  

作　　者：Zhong-e HOU Zhu LI Shu-qiang LI 

机构地区：[1]Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China [2]Beijing Museum of Natural History, Beijing 100050, China

出　　处：《Current Zoology》2009年第2期158-164,共7页动物学报（英文版）

基　　金：supported by the National Natural Sciences Foundation of China (NSFC-30499341/30770268/30870473);Beijing Financial Budget Index (2008-NO.0178);the Beijing Natural Science Foundation (5082013)

摘　　要：Using a standard cytochrome c oxidase I sequence,DNA barcoding has been shown to be effective to distinguish known species and to discover cryptic species. Here we assessed the efficiency of DNA barcoding for the amphipod genus Gammarus from China. The maximum intraspecific divergence for widespread species,Gammarus lacustris,was 3.5%,and mean interspecific divergence reached 21.9%. We presented a conservative benchmark for determining provisional species using maximum intraspecific divergence of Gammarus lacustris. Thirty-one species possessed distinct barcode clusters. Two species were comprised of highly divergent clades with strong neighbor-joining bootstrap values,and likely indicated the presence of cryptic species. Although DNA barcoding is effective,future identification of species of Gammarus should incorporate DNA barcoding and morphological detection.

关 键 词：GAMMARUS Cytochrome c oxidase I DNA barcoding Cryptic species TAXONOMY 

分 类 号：Q95[生物学—动物学]