^60Coγ射线离体照射诱导人线粒体COXI、ND1和ND6基因表达变化的研究  

Preliminary detection of expression changes of human mitochondrial COXI, ND1 and ND6 gene induced by ^60Co γ-rays

作　　者：李玉文[1] 封江彬[1] 陆雪[1] 陈德清[1] 刘青杰[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,北京100088

出　　处：《中华放射医学与防护杂志》2009年第2期125-128,142,共5页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金（30870749,30570551）;北京市自然科学基金（7053073）

摘　　要：目的探讨电离辐射诱导的人线粒体基因COXI、ND1和ND6表达的变化。方法用^60Coγ射线（1、3、5、8和10Gy）照射指数增长期的人淋巴细胞永生化细胞株，8h后用反转录PCR（RT-PCR）和实时荧光定量PCR（Real-timePCR）的方法检测线粒体基因（COXI、ND1和ND6）的表达变化，分析剂量-效应关系；以5Gy剂量照射后，于不同时点（0．5、4、8、12、24、48和72h）分析3种线粒体基因的表达变化，观察时间-效应变化。结果通过量效关系和时效关系方面的研究发现，COXI、ND1和ND6基因表达总体上调，但3种基因又具有其各自的特征：COXI基因除5Gy外，其他剂量照射后与对照组相比表达增强（F=116．62，P〈0．05）；ND1基因经1、8、10Gy剂量照射后，与对照组相比表达增强（F=25．13，P〈0．05），5Gy剂量照射后，除8和48h两组外，其余各组的表达改变与对照组相比，表达增强（F=223．68，P〈0．05）；ND6基因经1、8、10Gy剂量照射后与正常对照组比较，基因表达增强（F=18．51，P〈0．05），5Gy剂量照射后4、8、24、48和72h与对照组相比表达，明显增强（F=138．91，P〈0．05）。结论电离辐射可以诱导人线粒体COXI、ND1和ND6基因表达的改变，总体表达是增强的。Objective To explore the changes of human mitochondrial COXI, ND1 and ND6 genes expression induced by ionizing irradiation. Methods Changes of human COXI, ND1 and ND6 gene expression were detected by RT-PCR and Real-time PCR 8 h after the irradiation in human lymphoblastoid cell lines, which were exposed to 1-10 Gy ^60Co γ-rays. And the dose-effect relationships between expression changes of the genes and the doses were analyzed. The changes of these three genes expression were also analyzed at different post- radiation time-points between 0.5 h and 72 h after irradiation of 5 Gy in order to explore the time-effect. Results The expression of three genes COXI, ND1 and ND6, showed either the dose-effect or the time-effect after irradiation. The gene expression levels of three genes up-regulated generally and the peak change time-point was 4 h after irradiation. Conclusion Ionizing radiation might induce the changes of mitochondrial gene expression, and the gene expression level is up-regulated.

关 键 词：^60Coγ射线 人淋巴细胞系 线粒体 基因表达 

分 类 号：R817[医药卫生—影像医学与核医学]