肾蕨和镰叶肾蕨配子体发育及孢子繁殖研究  

Studies on Gametophyte Development and Spore Propagation of Nephrolepis auriculata and N. falcata

作　　者：刘保东[1] 檀龙颜[1] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,哈尔滨150025

出　　处：《园艺学报》2009年第4期545-552,共8页Acta Horticulturae Sinica

基　　金：国家‘863’计划项目(2007AA021405);国家科技基础条件平台项目(2005DKA21006);科技兴贸行动专项项目(2006GH559901);黑龙江省普通高等学校青年学术骨干支持计划项目(1152G015)

摘　　要：用无菌培养和常规繁殖方法培养肾蕨(Nephrolepisauriculata)和镰叶肾蕨(N.falcata)的孢子,对其配子体发育过程进行形态学观察,研究不同培养基中孢子的萌发率及成苗情况。结果表明,两种肾蕨均具有孢子单裂缝、成熟配子体为对称的心形或阔心形、毛状体单细胞、性器较小等共同特征。培养基中无机盐的浓度对孢子萌发及配子体发育状况影响较大。分析了3种含盐量不同的改良Knops固体培养基及基质培养基的优缺点,以及培养基中无机盐浓度和蔗糖浓度对这两种植物配子体产生的影响,表明蔗糖浓度低于2%利于孢子萌发。The spores of Nephrolepis auriculata and N. falcata were cultivated with sterile culture and routine propagation. The gametophyte development of the two species were observed morphologically. Spore germination rates and sporophyte appearance rates in various culture media and substrate were also studied. The results indicated the same characteristics of the gametophytes of the two species, such as the spores are all monolete, adult gametophytes are cordate or broad cordate, trichomes are unicellular and sex orgall is small. The rates of spore germination and gametophyte development of the two species were influenced heavily by concentration of mineral salt in various culture media. Advantages and disadvantages of the three improved Knop＇s solid culture medium and substrate culture medium were analysed respectively, also the influence of mineral salt concentration in various culture medium and sucrose concentration on gametophytes of the two species. That indicates the sucrose concentration under 2% is good for spore germination.

关 键 词：肾蕨 镰叶肾蕨 配子体 发育 孢子繁殖 

分 类 号：S682.35[农业科学—观赏园艺] Q945.51[农业科学—园艺学]