瑞典常春藤漆斑病病原鉴定  

Identification of the Pathogen Causing Black Spot Disease of Little Spurflower

作　　者：贲海燕[1,2] 李宝聚[1] 刘学敏[2] 石延霞[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]东北农业大学农学院,哈尔滨150030

出　　处：《园艺学报》2009年第4期553-558,共6页Acta Horticulturae Sinica

基　　金：国家科技基础条件平台建设项目(2005DKA21201);‘十一五’国家科技支撑计划项目(2006BAD07B02);科技部科研院所社会公益研究专项项目(2004DIB4J153)

摘　　要：通过病原菌的形态特征、培养性状和致病性测定的方法对引起瑞典常春藤漆斑病的病原菌进行了分离鉴定,采用PCR技术扩增病菌rDNA-ITS基因,获得一长度为523bp的DNA片段,序列测定结果表明该片段序列与GenBank已有的露湿漆斑菌序列的同源性达到了99%。综合两种方法鉴定结果,露湿漆斑菌是引起瑞典常春藤漆斑病的致病菌,露湿漆斑菌为该寄主上的真菌新病害。A pathogenic fungus causing leaf spot of Plectranthus parviflorus was isolated and characterized based on the morphological features, cultural characteristics and pathogenicity. By using polymerase chain reaction （PCR） technique to amplify rDNA-ITS of the isolate, we obtained a 523 bp DNA fragment, the result showed that rDNA-ITS sequences of the isolate and Myrothecium roridum shared 99% homology, which was compared in National Center for Biotechnology Information （NCBI） GenBank. Synthesized two identification results indicated that Myrothecium roridurn was the pathogen causing black spot disease of Plectranthus parviflorus. This is the first report of Myrothecium leaf spot caused by Myrothecium roridum on Plectranthus parviflorus.

关 键 词：瑞典常春藤 瑞典常春藤漆斑病 露湿漆斑菌 ITS-rDNA测序 

分 类 号：S682.36[农业科学—观赏园艺]