猪肠道寡肽转运载体1(PepT1)mRNA表达的肠段特异性和发育性变化  

Segmental Distribution and Ontogenetic Regulation of Peptide Transporter 1(PepT1) mRNA Expression in Intestine of Pigs

作　　者：邹仕庚[1,2] 冯定远[1] 黄志毅[1] 左建军[1] 职爱民[1] 董泽敏[1] 张常明[1] 叶慧[1] 

机构地区：[1]华南农业大学动物科学学院,广州510642 [2]广东科贸职业学院,广州510430

出　　处：《农业生物技术学报》2009年第2期229-236,共8页Journal of Agricultural Biotechnology

基　　金：国家重点基础研究发展规划(973)项目(No.2004CB117501);广东省科技攻关项目(No.2005B20201016)资助

摘　　要：研究旨在探讨猪肠道寡肽转运载体(PepT1)mRNA表达的肠段特异性和发育模式,为寡肽营养在养猪生产中的应用提供理论依据。实验分别选取遗传背景相同的1、7、26、30、60、90和150d蓝塘和长白公猪各5头,测体重后屠宰,取十二指肠、空肠、回肠和结肠组织样品;以18S rRNA为内标基因,用实时荧光定量PCR法(SYBR GreenⅠ试剂盒)检测PepT1 mRNA在60d长白猪表达的肠段特异性及其在蓝塘和长白猪肠道的发育模式。结果显示:长白猪肠道PepT1 mRNA的表达丰度十二指肠显著高于空肠和回肠(P<0.05),结肠无PepT1 mRNA的表达;PepT1 mRNA的表达丰度在十二指肠为蓝塘猪7、90d和长白猪60d,而在空肠和回肠为蓝塘猪60d和长白猪90d时达最高水平(P<0.05);蓝塘和长白猪肠道PepT1 mRNA的表达在断奶前(28d断奶)具有不同的发育模式,而在断奶后则具有相似的发育性变化;同一猪种不同肠段PepT1 mRNA的表达模式不同,但空肠和回肠在断奶后具有相似的发育性变化;不同猪种同一肠段PepT1 mRNA的表达蓝塘猪十二指肠在7和90d、空肠在7和60d及回肠在30d时分别显著高于长白猪(P<0.05)。结果说明猪肠道PepT1 mRNA的表达受到发育阶段、品种和肠段的调控。Segmental distribution and ontogenetic regulation of peptide transporter 1 （PepT1） mRNA expression were evaluated in pigs along the horizontal axis of the intestine. A total of 35 littermate purebred Lantang gilts and 35 litterrnate purebred Landrace gilts were divided into seven groups at the ages of day 1, 7, 26, 30, 60, 90 and 150, respectively. Intestinal segments （duodenum, jejunum, ileum and colon） were collected. The PepT1 mRNA abundance was determined by Real-time RT-PCR using SYBR Green Ⅰ RT-PCR mix Kit. Results showed that the abundance of PepT1 mRNA in duodenum was higher than in jejunum and ileum （P 〈0. 05）. There was no PepT1 mRNA expression in colon. The highest expression of PepT1 mRNA was on 7 and 90 d of Lantang and 60 d of Landrace in duodenum, 60 d of Lantang and 90 d of Landrace in jejunum and ileum, respectively （P 〈0.05）. The ontogenetic expression of PepT1 mRNA in intestine of Lantang and Landrace pigs was similarly during post-weaning （weaning on 28 d） period. The expression patterns of PepT1 mRNA in jejunum and ileum were similar after post-weaning. Abundance of PepTl mRNA in duodenum and jejunum of Lantang pigs on 7 and 90 d in duodenum, 7 and 60 d in jejunum and 30 d in ileum was higher than in corresponding segment of Landrace pigs. The results indicated that PepT1 mRNA expression can be regulated by developmental stage, breed and segment of intestine in pigs.

关 键 词：猪 肠道 寡肽转运载体1(PepT1)mRNA 肠段特异性 发育模式 

分 类 号：S188[农业科学—农业基础科学]