催乳素(PRL)和生长激素(GH)基因对高邮鸭产蛋性能的遗传效应分析  

Genetic Effects of Prolactin (PRL) and Growth Hormone (GH) Genes on Egg Performance in Gaoyou Duck

作　　者：李慧芳[1] 朱文奇[1] 宋卫涛[1] 张汤杰[2] 徐文娟[1] 陈宽维[1] 

机构地区：[1]中国农业科学院家禽研究所,扬州225003 [2]扬州大学兽医学院,扬州225009

出　　处：《农业生物技术学报》2009年第2期263-268,共6页Journal of Agricultural Biotechnology

基　　金：国家科技支撑计划项目(No.2008BADB2B08);江苏省高技术研究计划(No.BG2007323);江苏省科技服务平台(No.BM2008170);江苏省六大人才高峰项目资助

摘　　要：分别采用PCR-RFLP和PCR-SSCP方法检测催乳素(PRL)和生长激素(GH)基因的多态性,并分析了PRL和GH基因与高邮鸭产蛋性能的关系。结果表明:①PRL基因内含子1经酶切得出3种基因型AA/AB/BB,等位基因A/B频率分别为0.226/0.774;经测序发现PRL基因内含子1在1326bp处发生T→C突变。GH基因外显子4经SSCP分析显示3种基因型CC/CD/DD,等位基因C/D基因频率分别为0.222/0.778;经测序发现GH基因外显子4在3701bp处发生C→T突变。②PRL内含子1多态与高邮鸭的30周龄蛋重存在极显著关联(P<0.01);PRL多态与双黄蛋率显著关联(P<0.05);GH基因多态与最长连产天数和双黄率间相关性达显著性水平(P<0.05)。The polymorphisms ofprolactin （PRL） and growth hormone （GH） genes were detected using PCR-RFLP and PCR-SSCP, respectively. The association between the two genes （PRL and GH ） and egg performance of Gaoyou duck were analyzed. The resuits showed： （1）Three genotypes AA, AB and BB were found in PRL gene by PCR-RFLP. Frequencies of allele A and B were 0.226 and 0.774. A mutation T→C at the position of 1 326 bp （PRL intron 1） of the PRL gene in Gaoyou duck was found by DNA sequencing. Three genotypes CC, CD and DD were found in GH gene with the preponderant gene D frequency of 0.778 and the gene C frequency of 0.222. The mutations C→T at the position of 3 701 bp （GH extron 4） was found by DNA sequencing. （2）The highly significant association between PRL polymorphism and egg weight （30-weeks） was observed （P 〈0.01）. Three significant associations were found： for PRL and double-yolk percentage （P 〈0.05）, GH and the highest clutch days （P 〈0.05）, GH and double-yolk percentage （P 〈0.05）, respectively.

关 键 词：高邮鸭 催乳素(PRL) 生长激素(GH) 产蛋性能 

分 类 号：S188[农业科学—农业基础科学]