自然杀伤细胞免疫球蛋白样受体基因与白血病的关联性研究  

Association of killer immunoglobulin-like receptors with leukemia

作　　者：刘佳[1] 王全立[1] 刘广贤[2] 孙雪冬[2] 谭燕[2] 

机构地区：[1]解放军第307医院输血科,北京100071 [2]解放军第307医院血液科,北京100071

出　　处：《第三军医大学学报》2009年第9期794-796,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30672387;30772457)~~

摘　　要：目的分析自然杀伤细胞免疫球蛋白样受体(killer cell immunoglobulin-like receptor,KIR)基因与白血病的关联性。方法采用序列特异性引物PCR法(PCR-SSP)对46例白血病患者和46例健康人行KIR基因低分辨率检测,统计不同KIR基因在2种人群中的表现频率,并进行比较。结果健康人群和白血病患者中,2DL4、3DL2、3DL3、2DP1基因的表现频率均为100%,存在于所有个体,除2DL2与2DS2以外其余的KIR基因表现频率都相似;健康人群2DL2与2DS2的表现频率均为47.8%,白血病患者2DL2与2DS2的表现频率均为89.1%,明显高于健康人群(P<0.01),其余基因表现频率无显著性差异。结论2DL2、2DS2基因频率升高可能与白血病发生相关。Objective To study the correlation between the genes of killer immunoglobulin （Ig）-like receptors （KIR） in natural killer cells and leukemia. Methods Primers of 16 KIR genes, including KIR2DL1-5, KIR3DL1-3, KIR2DS1-5, KIR3DS1 and 2 pseudogenes 2DP1 and 3DPI, were designed and synthesized. A polymerase chain reaction with sequence-specific primers （PCR-SSP） was developed to detect the mRNA expressions of the 16 genes. Totally 46 healthy individuals and 46 leukemic patients （15 cases of acute myelocytic leukemia, 13 cases of chronic myelocytic leukemia and 18 cases of acute lymphocytic leukemia） were detected and the frequencies of the KIR genes were analyzed. Results The frequency of 2DIA, 3DL2, 3DL3 and 2DP1 in healthy and leukemic patients were 100%, and were detected in all patients and healthy indi- viduals. The other KIR genes had similar frequencies except those of 2DL2 and 2DS2, which were 47.8% of the healthy individuals and 89.1% in leukemic; patients. There was a significant difference between the 2 groups. Conclusion There may be an association between pathogenesis of leukemia and 2DL2 and 2DS2 genes.

关 键 词：杀伤细胞免疫球蛋白样受体 白血病 基因频率 

分 类 号：R392-33[医药卫生—免疫学] R392.13[医药卫生—基础医学]