熊果酸对DM大鼠主动脉血管NF-κB表达的影响  

Effects of ursolic acid on the expression of nuclear factor-κB in the aorta of diabetic rat

作　　者：王建梅[1] 向敏[2] 凌婧[1] 徐晓月[3] 

机构地区：[1]苏州卫生职业技术学院药学系,苏州215009 [2]苏州卫生职业技术学院生物技术中心,苏州215009 [3]河南省食品药品检验所药理室,郑州450003

出　　处：《中药药理与临床》2009年第1期11-14,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：江苏省卫生厅立项课题(H200764)

摘　　要：目的:观察熊果酸(Ursolicacid,UA)保护糖尿病(DM)大鼠血管病变的作用,并探讨其对NF-κB蛋白表达的影响。方法:以STZ诱导DM大鼠模型,设DM模型组、UA两剂量治疗组(50mg/kg,25mg/kg)和氨基胍治疗组(100mg/kg),同时设正常对照组。给药8wk后,用血糖仪测定空腹血糖,试剂盒测定血清MDA和NO水平,HE染色观测主动脉的病理变化;同时,采用sABC法检测核转录因子(NF-kBp65)蛋白的表达。结果:UA50mg/kg治疗后DM大鼠血糖值降低,血清MDA和NO水平较模型组降低;病理切片和免疫组化结果显示UA治疗组血管损伤较模型减轻且NF-κB阳性表达明显低于模型组。结论:UA有保护DM大鼠血管损伤的作用,机制可能与UA抗氧化,从而抑制NF-kB的激活有关。AIM ： The protection of ursolic acid for the aorta of diabetic rat and its effect on the expression of nuclear factor-KB were studied. Methods： Rats were intravenous injected with STZ at the dose of 60mg/kg. 3 days later, the rats whose blood glucose were over 11.1 mmol/L were regards as diabetic rats and were divided into 4 groups randomly： diabetic mellitus （DM） group, UA （50mg/kg and 25mg/kg） group, amgudine （100mg/kg） group. Normal group was arranged simultaneously. After been treated for 8 weeks, the levels of fasting blood glucose （FBC） , serum MDA and NO were measured. The pathohistology of aortic arch was examined and the positive expression of NF-κB in the aorta was tested by immunohistochemistry. Results： The levels of fast blood glucose（ FBG）, serum MDA and NO of UA group （50mg/kg） decreased and there were significant compared with DM group （ P 〈 0.01 ）. HE staining demonstrated the injury of aorta was attenuated and the activation of NF-κB was inhibited in UA group. Conclusion： The protection of ursolic acid for the aorta of diabetic rat should be related to the inhibition to reactive oxide stress （ROS） and activation of NF-κB and attenuate diabetic vascular complications in DM rats.

关 键 词：熊果酸 糖尿病 核转录因子-ΚB 血管病变 

分 类 号：R285[医药卫生—中药学]