莲心碱对大鼠脑缺血／再灌注后TNF-α表达的影响  

Effect of liensinine on TNF-α expression on focal cerebral ischemia-reperfusion injury in rats

作　　者：余万桂[1] 邓德明[1] 陈庭煊[2] 张恒文[1] 张燕祥[1] 张道明[1] 刘莲[1] 

机构地区：[1]长江大学医学院,荆州434000 [2]荆州市第一人民医院,荆州434000

出　　处：《中药药理与临床》2009年第1期16-18,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：湖北省卫生厅科研基金资助项目(NO:JXzb74)

摘　　要：目的:观察莲心碱对大鼠脑缺血/再灌注后TNF-a表达的影响并探讨其作用机制。方法:采用大鼠大脑中动脉线栓(MCAO)法制备局灶性脑缺血/再灌注模型,将90只SD雄性大鼠随机分为3组:假手术组、缺血再灌注组和莲心碱组,应用免疫组化法分别检测各组大鼠缺血/再灌后TNF-a的动态变化并进行图像分析。结果:与假手术组比较,缺血再灌注组大鼠TNF-a的表达于脑缺血/再灌注3h已有明显升高,24h达到高峰。与损伤对照组比较,莲心碱组大鼠TNF-a的表达24h减少。结论:莲心碱的神经保护机制可能与抑制TNF-a的表达有关。Objective： Observe the effect of Liensinine on TNF-α expression on focal cerebral ischemia-reperfusion injury in rats. Methods： The model of focal cerebral ischemia-reperfusion injury in rats was made by suture-occluded method . 90 male Sprague-Dawley rats were randomly divided into three groups： sham operation group, ischemia-reperfusion group, liensinine group, lmmunochemistry were applied to detect dynamic diversification TNF-α and make image analysis. Results： The expression of TNF-α increased at 3 h ＇after reperfusion, reached apex at 24h and liensinine significantly inhibited the expression of TNF-α at 24h. Conclusion ： The protective mechanism of liensinine is probably related to the inhibition of TNF-α expressionl .

关 键 词：莲心碱 脑缺血/再灌注 TNF—α 

分 类 号：R285[医药卫生—中药学]