五灵脂抗动脉粥样硬化炎症作用研究  

Study on anti-inflammatory effect of Faces Trogopterus (五灵脂) in experimental arterosclerotic rats

作　　者：唐绪刚[1] 黄文权[1] 姜利鲲[1] 

机构地区：[1]第三军医大学新桥医院康复理疗中心,重庆400037

出　　处：《中药药理与临床》2009年第1期35-38,共4页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:观察五灵脂(Faeces Trogopterorum,FT)水提取物对实验性动脉粥样硬化(artherosclerosis,AS)大鼠细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)表达及动脉内膜病变的影响,探讨FT抗AS炎症作用的机制。方法:采用高脂饮食结合腹腔注射维生素D3注射液复制动脉粥样硬化模型,模型复制成功后给予FT水提取物2.5g/kg、10g/kg灌胃干预。用药6周后采用免疫组化技术观察主动脉ICAM-1表达,反转录聚合酶链反应(reverse transcriptase-polymerase chain reaction,RT-PCR)检测主动脉ICAM-1 mRNA表达,透射电镜观察主动脉内膜病变情况。结果:同模型组比较,五灵脂水提取物能抑制大鼠主动脉ICAM-1及ICAM-1 mRNA表达,高剂量作用较低剂量作用明显。电镜显示FT组大鼠动脉病变轻于模型组。结论:FT能降低实验性动脉粥样硬化大鼠ICAM-1表达,减轻血管内皮病变程度,这可能是其抗AS炎症的分子机制之一。Objective ：To explore the mechanism of Faces Trogopterus （FT） anti-atherogenesis inflammatory through observing the effects of water extracts of FT on the expression of intercellular adhesion molecule-1 in the experimental artherosclerosis rats. Methods ： Sixty healthy male clean Wistar rats were randomly divided into 4 groups of 15 rats per group： model group,control group, FT I group（ FT I ） ,FT Ⅱ group（ Ⅱ ）. The control group was fed standard feedstuff. The model, FT I and Fr Ⅱ group were duplicated by feeding high cholesterol diet and peritoneal injecting vitamin D3. Ten weeks later, FT I and FT Ⅱ group were interfered by intragastric administration with different dose（2.5 g/ kg, 10 g/kg） of the water extracts of FT for six weeks. At last, the expression of intercellular adhesion molecule-1 proteinum and mRNA in the endarterium of arterosclerotic rats were detected by immunohistochemical technique and reverse transcriptasc-polymerase chain reaction; the pathological changes in the endarterium of arterosclerotic rats was observed by transmission electron microscope. Results： The AS model was successfully duplicated. Compared with the model group, the expression of intercellular adhesion molecule-1 proteinum and mRNA in the end- arterium of the rats was significantly lower（ P 〈 0.01 or P 〈 0.05 ）. Transmission electron microscope result was that the endothelial damage of the aortas in FT I and FT Ⅱ groups was less severe than that in model group. Conclusions： FT can downregnalte the expression of ICAM-1 in the artery of experimental arterosclerotic rats, which may account for the anti-arteriosclerosis inflammatory effects of FT.

关 键 词：五灵脂 动脉粥样硬化 细胞间黏附分子-1 

分 类 号：R285[医药卫生—中药学]