苦参碱对胶质瘤大鼠模型中Fas表达的调节作用的实验研究  

Effect of matrine on Fas expression in C6 glioma in rats

作　　者：戚基萍[1] 刘微[1] 张淑君[2] 昝丽坤[1] 孙玉兰[1] 宋月佳[3] 

机构地区：[1]哈尔滨医科大学第一临床学院病理科,哈尔滨150001 [2]黑龙江省医院病理科,哈尔滨150001 [3]哈尔滨医科大学,哈尔滨150001

出　　处：《中华神经医学杂志》2009年第4期355-358,共4页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（30470661）

摘　　要：目的探讨苦参碱应用前后C6脑胶质瘤大鼠模型中Fas因子的表达变化及意义。方法采用脑立体定向技术，将体外培养的C6胶质瘤细胞注入大鼠尾状核区制备胶质瘤大鼠模型，并根据是否用药及用药量的多少分为空白对照组、冰片组、苦参碱低剂量组、苦参碱高剂量组、苦参碱低剂量＋冰片组、苦参碱高剂量＋冰片组。通过大鼠生存状态、标本的大体所见、MRI、HE染色观察苦参碱对胶质瘤大鼠模型生存质量及胶质瘤体积的影响，用免疫组织化学方法检测苦参碱对胶质瘤大鼠模型肿瘤细胞中Fas表达的影响。结果大鼠生存状态、标本的大体所见、MRI及HE染色显示苦参碱可显著提高胶质瘤大鼠模型的生存质量，抑制胶质瘤细胞增殖。免疫组化结果显示，苦参碱低剂量＋冰片组（98．16±11．82）、苦参碱高剂量＋冰片组（112．80±12．12）Fas表达高于空白对照组（39．09±7．79）、冰片组（46．87±7．43）、苦参碱低剂量组（42．41±7．83）、苦参碱高剂量组（44．20±7．47），苦参碱高剂量＋冰片组Fas表达高于苦参碱低剂量＋冰片组，差异均有统计学意义（P〈0．05）。结论苦参碱能增加胶质瘤细胞中Fas的表达，抑制胶质瘤细胞增殖。Objective To investigate the effect ofmatrine on Fas expression in C6 glima in a tumor-bearing rat model. Methods Cultured cerebral glioma C6 cells were injected stereotactically into the left caudate nucleus of the rats. The rats were randomized into untreated group, bomeol-treated group, low-dose matrine group, high-dose matrine group, low-dose matrine ＋ borneol group, and high-dose matrine ＋ bomeol group. The effect ofmatrine on the quality of life of the rats and the glioma volume was evaluated according to the survival state of the rats and by gross observation, magnetic resonance imaging （MRI） and HE staining of the brain tissue. Immunohistochemistry was performed to detect Fas expression in the glioma cells. Results The survival state of the rats, gross observation of the brain specimen, and results of MRI and HE staining all showed that matrine significantly improved the quality of life of the glioma-bearing rats and inhibited the glioma cell proliferation. Fas expression was significantly higher in low-dose matrine ＋ bomeol group （98.16±11.82） and high-dose matrine ＋ bomeol group （112.80±12.12） than in untreated group （39.09±7.79）, bomeol group （46.87±7.43）, low-dose matrine group （42.41±7.83）, and high-dose matrine group （44.20±7.47）（P〈0.05）. Fas expression was obviously upregulated in the high-dose matrine ＋ borneol group as compared with the low-dose matrine ＋ bomeol group （P〈0.05）. Conclusion Matrine can significantly upregulate Fas expression in glioma and inhibit glioma cell proliferation in the glioma-bearing rat model..

关 键 词：苦参碱 神经胶质瘤 FAS 细胞凋亡 

分 类 号：R686[医药卫生—骨科学]