萝卜花粉培养及自交育性检测  

Pollen in vitro Culture and Viability Detection of Self-pollen on Radish

作　　者：刘春香[1] 刘明[1] 赵光强[1] 陈爱军[1] 

机构地区：[1]潍坊学院生物工程学院,山东潍坊261061

出　　处：《北方园艺》2009年第4期23-26,共4页Northern Horticulture

基　　金：国家星火计划资助项目(2007EA740101)

摘　　要：对18种固体培养基进行筛选,得出蔗糖10%、琼脂0.5%、硼酸0.02%和蔗糖5%、琼脂0.3%、硼酸0.04%两种配方,在温度25℃,相对湿度大于95%,时间在4h是培养萝卜花粉的适宜条件,花粉萌发率达97%以上。通过自花授粉、显微镜观察及苯胺蓝染色紫外灯箱观察花柱中花粉管生长状况相结合来分析花粉在自花柱头上的育性,对花粉活力还进行了化学染色方面的探索。结果表明:0.1%苯胺蓝染液可以准确分析花粉管能否穿越自花柱头,从而鉴定自交亲和性,而碘化钾染色法不适合检测萝卜花粉活力。The two optimum pollen solid culture medium of radish were selected from 18 medium, under the condition of relative humidity above 95%, at 25℃, 4 hours growing time. One was sucrose 10%, agar 0. 5%, and boric acid 0.02 %, the other was sucrose 5%, agar 0.3%, and boric acid 0.04%, the pollen germination rate ,were more than 97% under these conditions. This study analyzed pollen fertility by combining self-pollination with observing the tube growth state in the stylous under microscope and under UV light by 0. 1 % aniline blue to tests the viability of the radish pollen by staining. Chemical staining method was tried to detect pollen viability, too. The result showed that 0. 1 % aniline blue staining can detect whether self-pollen tube can pass through their chapiter for self-incompatibility analysis, and potassium iodide was not fit for radish pollen viability detection.

关 键 词：萝卜 花粉培养 花粉活力 花粉育性 检测 

分 类 号：S631.103.6[农业科学—蔬菜学]