不同培养基及糖浓度对丰花月季叶愈伤组织诱导的影响  

The Effects of Different Media and Sugar Concentrations on Callus Induction from Leaflet of Rosa chinensis

作　　者：赵松峰 

机构地区：[1]河南省烟草技工学校,河南许昌461000

出　　处：《北方园艺》2009年第4期56-58,共3页Northern Horticulture

基　　金：河南省科技攻关资助项目(0624050023)

摘　　要：以丰花月季叶片为材料,在MS,B5,White,WPM4种基本培养基和在含不同糖浓度为0、30、60、90g/L的MS培养基上,进行愈伤组织的诱导。结果表明:4种培养基中,MS培养基的诱导率最高,4种糖浓度中,以糖浓度为30g/L的诱导率最高。最佳培养基为MS+6-BA0.1mg/L+NAA0.1mg/L+糖30g/L+琼脂7.5g/L.Rosa chinensis leaflet were treated in MS, Bs, White, the WPM medium and added sugar in MS medium with different concentration, 0 g/L, 30 g/L, 60 g/L, 90 g/L. Callus induction were studied. The result indicated that the induction rate was highest in MS medium, in 30 g/L sugar; The best medium was MS＋6-BA 0. 1 mg/L＋NAA 0. 1 mg/L＋sugar 30 g/L＋agar 7. 5 g/L.

关 键 词：月季 培养基 愈伤组织 组织培养 

分 类 号：S685.12[农业科学—观赏园艺]