水芋体细胞胚胎发生及植株再生体系的建立  

Establishment of in vitro Somatic Embryogenesis and Plant Regeneration System of Calla palustris L.

作　　者：刘秀岩[1,2] 顾地周[2] 朱俊义[2] 梁宇[1,2] 

机构地区：[1]北华大学林学院,吉林吉林132013 [2]通化师范学院生物系,吉林通化134002

出　　处：《北方园艺》2009年第4期62-65,共4页Northern Horticulture

基　　金：国家科技部“国家科技攻关计划引导资助项目”(2005BA741C)

摘　　要：以水芋幼叶为外植体,应用均匀设计法筛选其最适合的胚性愈伤组织及胚性细胞复合体诱导、体细胞胚胎发育及植株再生的培养基,建立了水芋体细胞胚胎发生体系。对不同阶段培养材料的形态结构及超微结构的观察证明了水芋体细胞胚胎的发育过程。结果表明:水芋叶片胚性愈伤组织及胚性细胞复合体诱导最适宜培养基为:LS+6-BA0.5mg/L+2,4-D2.0mg/L,诱导率为98.5%;体胚发育及植株再生最适宜的培养基为:LS+6-BA1.0mg/L+NAA0.5mg/L+IAA1.0mg/L,体胚萌发率为96%,萌发的体胚在发育培养基上继续培养25d后全部发育成完整植株。Young leaves of Calla palustris L. were used as explants in this experiment. Uniform Dedign for the most suitable media for Embryogenic callus induction and Embryogenic cell complex,Development of Somatic embryo and plant regeneration were screened, in vitro plants has been successfully developecL The results showed that LS＋6-BA 0. 5 nag· L-1 ＋2,4-D 0. 2 mg· L-1 was fit for Embryogenic callus and Embryogenic cell complex induction, Percentage was 98. 5%; the medium of Development of Somatic embryo and plant regeneration was LS＋6-BA 1. 0 mg · L-1 ＋NAA 0. 5 mg ·L-1 ＋IAA 1. 0 mg · L-1, Percentage was 96%, and converted into plantlets with shoots and roots after 25 days culture on the same medium. The obervation of and ultrastructure proved the process of somatic embryogenesis of Calla palustris L.

关 键 词：水芋 均匀设计 胚性细胞复合体 体细胞胚 植株再生 

分 类 号：S567.239[农业科学—中草药栽培]