中药脊髓康治疗大鼠脊髓损伤的实验研究  

Experiment study of Jisuikang on treatment of rat after spinal cord injury

作　　者：周建中[1] 马勇[2] 殷韶健[1] 孙士杰[1] 

机构地区：[1]南京中医药大学,2006级硕士研究生南京210029 [2]南京中医药大学第一临床医学院骨伤科研究所,南京210029

出　　处：《江西中医学院学报》2009年第2期71-74,共4页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：江苏省中医药局资助项目(HD5072)

摘　　要：目的:观察脊髓康对脊髓损伤大鼠的治疗作用及其作用是否有剂量依赖性,并与强的松进行对照。方法:SD大鼠72只随机分为6组(n=12)。造模后脊髓康高、中、低剂量组予脊髓康25、12.5、6.25g生药/kg体重;强的松组予强的松0.06g/kg体重。正常组及模型组予等量蒸馏水。测定血液及损伤脊髓中IL-10、INF-α、NO、NOS、SOD、MDA含量;全血粘度;损伤脊髓病理学检查。结果:血液中TNF-α含量升高,脊髓康各剂量组相对模型组降低。血液中IL-10水平下降,强的松组和脊髓康各剂量组相对模型组有显著差异。强的松组相对模型组全血比粘度无差异。脊髓康高、中剂量组低于模型组。血液SOD活力下降,MDA、NO和NOS含量升高。脊髓康高、中剂量组SOD活力高于模型组,MDA及NO含量低于模型组。脊髓康各剂量组NOS含量低于模型组。强的松组相对模型组MDA含量下降,SOD活性无明显变化。脊髓损伤部位SOD活力下降,MDA、NO和NOS含量升高。脊髓康高剂量组SOD活力高于模型组。脊髓康高、中剂量组MDA、NOS含量低于模型组。脊髓康各剂量组NO含量低于模型组。强的松组相对模型组MDA含量下降,SOD活性无明显变化。病理结果:脊髓康高剂量组脊髓病变程度较轻。中剂量组较高剂量组稍差。低剂量组白质区水肿。强的松组与高、中剂量组相似。结论:脊髓康能抑制NOS表达、降低NO含量;能降低MDA含量、提高SOD活性;能改善血液流变学指标;能降低TNF-α水平;能提高IL-10水平;能防止脊髓损伤的继发性改变,促进脊髓组织恢复,促进神经组织再生,高剂量较中剂量疗效更显著。Objective： To observe the effect of Jisuikang on treatment of rats after empirical spinal cord injury, and understand whether has dose-dependent effect or not, and perform positive control with prednisone. Methods： Seventy-two SD rats were separated randomly into six groups （n = 12）. The rats in the Jisuikang high-dose group, middle-dose group and low-dose group were given Jisuikang 25,12. 5,6.25 g/kg for 14 days. The rats in the prednisone group were given 0.06 g/kg prednisone for 7 days. The rats in normal and model group were given distilled water of the same volume. After one hour of last administration, collecting the blood in rats＇ fossa orbitalis of every group,testing the content of IL-10,TNF-α and NO,SOD,NOS,MDA in the blood; testing whole blood viscosity; testing the content of NO,SOD,NOS,MDA in the injuried spinal cord tissue. Spinal cord histopathologic examination was done ater determination. Results ： After modeled, the content of TNF-α in the blood of rats was raised compared with normal group, and this in Jisuikang high-dose group（ P 〈 0.01 ）, middle-dose group（ P 〈 0.05 ） and low-dose group（P 〈 0.01 ） was decreased compared with model group. After modeled the content of IL-10 in the blood of rats was obviously decreased compared with normal group, and this in Jisuikang high-dose group, middle-dose group, low-dose group and prednisone group was significant difference as compared with model group （P 〈 0.01 ）. After modeled, the rats＇ whole blood viscosity was significant difference as compared with normal group. And this in prednisone group had no difference as compared with model group. But this in Jisuikang high-dose group and middle-dose group was lower than this in model group. After modeled,the content of SOD in the blood was obviously decreased but MDA, NO and NOS raised. The content of SOD in Jisuikang high-dose group and middle-dose group was higher than this in model group, but the MDA content in the two groups was lower than this in model group. The

关 键 词：脊髓损伤 脊髓康 中药 实验研究 

分 类 号：R285.5[医药卫生—中药学]