丰城鸡血藤芒柄花素提取工艺研究  

The Extraction Process Study of Formononetin in Millettla nitida var hirsutissima Z wei

作　　者：钟小群[1] 李艳[1] 王丽静[1] 利家平[1] 朱良辉[1] 徐丽瑛[1] 李良[1] 

机构地区：[1]江西省药物研究所,南昌330029

出　　处：《江西中医学院学报》2009年第2期77-78,共2页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：国家科技支撑计划子课题(2006BAI06A18-09);江西省科技计划任务

摘　　要：目的:对丰城鸡血藤有效成分芒柄花素的提取工艺进行研究。方法:采用HPLC法测定不同提取工艺及纯化工艺中芒柄花素含量,计算提取率。结果:芒柄花素的稀醇提取率大于水提取率,但是稀醇提取物复溶性差,产生大量黑色胶状、絮状及焦屑样的沉淀物;提取液滤去沉淀物后,其保留率小于纯化后的水提取物。结论:芒柄花素的稀醇溶解度大于水溶解度。药材可以采用稀醇提取。但是醇提取物纯化后和水提取结果相似。Objective：To study the extraction and purification processes of Formononetin in Millettla nitida var hirsutissima Z wei. Method ： HPLC was used to determinate the consent of Formononetin in different extractions or purified extractions, by which the best method was chosen. Result ： The extraction rate of Formononetin in water-alcohol was bigger than that in water, But the re-solubility of the former were not good with a large number of dark Jelly ,flocculation and black Coke crumbs kind of sediments and had almost the same retention rate as that of the latter after filtered sediments. Conclusion ： The dissolution of Formononetin in water-alcohol was bigger than that in pure water,the herb can use water-alcohol as its extraction solvent but with the same purified rate as that of water.

关 键 词：丰城鸡血藤 HPLC 芒柄花素 提取方法 

分 类 号：R284.2[医药卫生—中药学]